Micellar electrokinetic chromatography with laser induced fluorescence detection shows increase of putrescine in erythrocytes of Parkinson's disease patients
Micellar electrokinetic chromatography with laser induced fluorescence detection shows increase of putrescine in erythrocytes of Parkinson's disease patients
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DOI：
10.1016/j.jchromb.2018.02.015
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发表时间：
2018-04-01
影响因子：
3
通讯作者：
Paredes, D. A.
中科院分区：
文献类型：
--
作者：
Betancourt, L.;Rada, P.;Paredes, D. A.
建立了一种高灵敏度的腐胺胶束电动色谱-激光诱导荧光检测方法，在1 nM ~ 30 n范围内线性良好。这项技术在帕金森病患者的一滴血上进行了测试，这滴血是通过针刺手指获得的。结果显示，与对照组相比，红细胞中腐胺的增加具有统计学显著性，而血浆中腐胺的增加不显著。这种高水平的腐胺本身并不能证明腐胺和多胺与帕金森病直接相关。然而，目前的结果和其他几个在讨论中提出的建议，这些化合物可能是因果关系参与帕金森氏病的病理生理。此外，本文报道的分析方法可能有助于寻找包括帕金森病在内的许多疾病的新生物标志物。
A highly sensitive method was developed to measure putrescine by micellar electrokinetic chromatography with laser induced fluorescence detection with excellent linearity in the 1 nM to 3 On range. The technique was tested on a drop of blood from Parkinson's disease patients obtained by finger prick. The results showed a statistically significant increase of putrescine in the erythrocytes compared to controls and a non-significant increase in plasma. This high level of putrescine does not constitute by itself proof that putrescine and polyamines are directly related to Parkinson's disease. However, the present results and several others addressed in the discussion suggest that these compounds might be causally involved in the pathophysiology of Parkinson's disease. In addition, the analytical method reported here may help to find new biomarkers for many diseases including Parkinson's disease.