EMT-related gene expression is positively correlated with immunity and may be derived from stromal cells in osteosarcoma
EMT-related gene expression is positively correlated with immunity and may be derived from stromal cells in osteosarcoma
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DOI：
10.7717/peerj.8489
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发表时间：
2020-02-03
期刊：
影响因子：
2.7
通讯作者：
Quan, Zheng-xue
中科院分区：
文献类型：
--
作者：
Peng, Yin-xiao;Yu, Bin;Quan, Zheng-xue
背景：骨肉瘤是儿童和年轻人最常见的骨癌类型。最近的研究表明，上皮间质转化（EMT）相关基因的表达和免疫在人类癌症之间的相关性。在这里，我们调查EMT，免疫活性，基质活性和肿瘤purity在osteosarcoma.Methods之间的关系：我们定义EMT基因签名和评估免疫活性和基质活性的基因表达和临床数据的基础上，从三个独立的芯片数据集。通过单样本基因集富集分析和ESTIMATE工具评估这些因素。最后，我们分析了EMT基因表达的关键来源，从基因表达的Omnibus和人类样本，我们收集的microarray datasets。结果：EMT相关基因的表达与骨肉瘤中的免疫和基质活性呈正相关。肿瘤纯度与EMT、免疫活性和基质细胞呈负相关。我们进一步证明，高EMT基因表达可以显着预测骨肉瘤患者的总生存期（OS）和无复发生存期（RFS），而高免疫活性不能。然而，结合这些因素可能对骨肉瘤患者的OS和RFS有进一步的预后价值。最后，我们发现间质细胞可能是骨肉瘤中EMT基因表达的一个关键来源。结论：本研究结果显示，EMT基因的表达与免疫呈正相关，但这些特征传达了不同的预后信息。此外，结果表明，EMT相关基因的表达可能来自间质而不是上皮癌细胞。
Background: Osteosarcoma is the most common type of bone cancer in children and young adults. Recent studies have shown a correlation between epithelial- mesenchymal transition (EMT)-related gene expression and immunity in human cancers. Here, we investigated the relationship among EMT, immune activity, stromal activity and tumor purity in osteosarcoma.Methods: We defined EMT gene signatures and evaluated immune activity and stromal activity based on the gene expression and clinical data from three independent microarray datasets. These factors were evaluated by single sample Gene Set Enrichment Analyses and the ESTIMATE tool. Finally, we analyzed the key source of EMT gene expression in osteosarcoma using microarray datasets from the Gene Expression Omnibus and human samples that we collected.Results: EMT-related gene expression was positively correlated with immune and stromal activity in osteosarcoma. Tumor purity was negatively correlated with EMT, immune activity and stromal cells. We further demonstrated that high EMT gene expression could significantly predict poor overall survival (OS) and recurrence-free survival (RFS) in osteosarcoma patients, while high immune activity cannot. However, combining these factors could have further prognostic value for osteosarcoma patients in terms of OS and RFS. Finally, we found that stromal cell s may serve as a key source of EMT gene expression in osteosarcoma.Conclusion: The results of this study reveal that the expression of EMT genes and immunity are positively correlated, but these signatures convey disparate prognostic information. Furthermore, the results indicate that EMT-related gene expression may be derived from stromal rather than epithelial cancer cells.