Characterization of scallop midgut gland certified reference material for quantification of diarrhetic shellfish toxins
Characterization of scallop midgut gland certified reference material for quantification of diarrhetic shellfish toxins
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用于定量腹泻性贝类毒素的扇贝中肠腺认证标准物质的表征
DOI：
10.1016/j.foodchem.2019.125011
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发表时间：
2019
期刊：
影响因子：
8.8
通讯作者：
Suzuki Toshiyuki
中科院分区：
文献类型：
--
作者：
Yarita Takashi;Inagaki Shinsuke;Miyamoto Ayano;Yamazaki Taichi;Kawaguchi Migaku;Uchida Hajime;Takatsu Akiko;Suzuki Toshiyuki
开发了一种扇贝中肠腺认证标准物质NMIJ CRM 7520-a，用于贝类腹泻性贝类毒素检验过程中的验证和质量保证。候选材料是用天然毒性和无毒的煮沸中肠腺加冈田酸（OA）制备的。发现材料的均匀性和稳定性是适当的。为了表征OA和dinophysistoxin-1 (DTX1)， 9名参与者参与了基于日本官方测试方法的联合实验室研究，其中化合物在碱性水解后采用液相色谱-串联质谱法进行分析。分析值采用标准加样法获得，标准加样液采用标准溶液认证标准物质OA和DTX1校准。确定了扩展不确定度（覆盖因子= 2，约95%置信区间）的认证浓度，OA为（0.205 ± 0.061）mg/kg， DTX1为（0.45 ± 0.11）mg/kg。
A scallop midgut gland certified reference material, NMIJ CRM 7520-a, was developed for validation and quality assurance during the inspection of shellfish for diarrhetic shellfish toxins. The candidate material was prepared by using naturally-toxic and nontoxic boiled midgut glands spiked with okadaic acid (OA). The homogeneity and stability of the material were found to be appropriate. For the characterization of OA and dinophysistoxin-1 (DTX1), nine participants were involved in a co-laboratory study based on the Japanese Official Testing Method, where the compounds were assayed by liquid chromatography-tandem mass spectrometry following alkaline hydrolysis. The analytical values were obtained by the standard addition method with a standard spiking solution calibrated using the standard-solution certified reference materials OA and DTX1. The certified concentrations with expanded uncertainties (coverage factork= 2, approximate 95% confidence interval) were determined to be (0.205 ± 0.061) mg/kg for OA and (0.45 ± 0.11) mg/kg for DTX1.