ELECTRON MICROSCOPIC STUDIES OF MITOSIS IN AMEBAE .1. AMOEBA-PROTEUS
ELECTRON MICROSCOPIC STUDIES OF MITOSIS IN AMEBAE .1. AMOEBA-PROTEUS
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DOI：
10.1083/jcb.8.1.207
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发表时间：
1960-01-01
期刊：
影响因子：
--
通讯作者：
DANIELS, EW
中科院分区：
文献类型：
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作者：
ROTH, LE;OBETZ, SW;DANIELS, EW
变形阿米巴的单个生物体被固定在含有0.9%氯化钠或0.01%氯化钙的四氧化三铯缓冲液中，切片，并在有丝分裂的间期和几个阶段进行电子显微镜研究。间期核的典型螺旋不与浓缩的染色质共存，因此要么代表间期特有的DNA构型，要么根本不是DNA。复合核被膜在观察到的所有阶段都存在，但在间期是不连续的。核被膜内厚的蜂窝层在核重建的前期消失，末期后重新出现。核仁的大小和数量在前期减少，在末期重新形成，与染色质网络有关。在早期的重建核中，核仁物质形成薄的片状结构，与少量的染色质密切相关，并紧密应用于核膜的内层，部分形成的层。有人提出，核仁物质与包膜内层的形成有关，并且有一种核仁物质的构型是这次所特有的。在分裂过程中，质膜的流苏状物质被部分剥离。
Individual organisms of Amoeba proteus have been fixed in buffered osmium tetroxide in either 0.9 per cent NaCl or 0.01 per cent CaCl2, sectioned, and studied in the electron microscope in interphase and in several stages of mitosis. The helices typical of interphase nuclei do not coexist with condensed chromatin and thus either represent a DNA configuration unique to interphase or are not DNA at all. The membranes of the complex nuclear envelope are present in all stages observed but are discontinuous in meta-phase. The inner, thick, honeycomb layer of the nuclear envelope disappears during prophase, reappearing after telophase when nuclear reconstruction is in progress. Nucleoli decrease in size and number during prophase and re-form during telophase in association with the chromatin network. In the early reconstruction nucleus, the nucleolar material forms into thin, sheet-like configurations which are closely associated with small amounts of chromatin and are closely applied to the inner, partially formed layer of the nuclear envelope. It is proposed that nucleolar material is implicated in the formation of the inner layer of the envelope and that there is a configuration of nucleolar material peculiar to this time. The plasma-lemma is partially denuded of its fringe-like material during division.