Engineering a chemoenzymatic cascade for sustainable photobiological hydrogen production with green algae
Engineering a chemoenzymatic cascade for sustainable photobiological hydrogen production with green algae
复制标题
设计化学酶级联以利用绿藻实现可持续光生物制氢
DOI：
10.1039/d0ee00993h
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发表时间：
2020-07-01
影响因子：
32.5
通讯作者：
Fan, Chunhai
中科院分区：
文献类型：
--
作者：
Chen, Jie;Li, Jiang;Fan, Chunhai
光生生物制氢是大规模生产氢能的最有前途的途径之一。然而，光生物方法的成本和可持续性在很大程度上阻碍了其大规模商业化生产。在这里，我们设计了一个由葡萄糖、葡萄糖氧化酶、过氧化氢酶和氢氧化镁组成的强大的化学酶级联系统，设计了一个具有恒定近中性pH的厌氧环境来持续诱导绿藻的絮凝。我们发现衣藻在该工程光生物系统中能稳定产氢近一个月，平均产氢速率为0.44mmolH(2)h(-1)(mg叶绿素)(-1)。因此，这项研究为工业规模的光生物制氢开辟了一条新的途径，具有广阔的“液体阳光”应用前景。
Photobiological hydrogen generation is among the most promising routes for the mass production of hydrogen energy. However, the cost and sustainability of photobiological methods largely hamper their large-scale commercial production. Here, we design an anaerobic environment with a constant near-neutral pH for the sustainable induction of green algae flocculation by engineering a robust chemoenzymatic cascade system consisting of glucose, glucose oxidase, catalase, and magnesium hydroxide. We found thatChlamydomonas reinhardtiicould stably produce hydrogen in this engineered photobiological system for nearly a month, with an average rate of 0.44 mu mol H(2)h(-1)(mg chlorophyll)(-1). This study thus opens a new avenue to photobiological hydrogen production at industrial scales for promising "liquid sunshine" applications.