Innate lymphoid cells regulate radiation-induced skin damage via CCR10 signaling
Innate lymphoid cells regulate radiation-induced skin damage via CCR10 signaling
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先天淋巴细胞通过 CCR10 信号传导调节辐射引起的皮肤损伤
DOI：
10.1080/09553002.2020.1793013
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发表时间：
2020
影响因子：
2.6
通讯作者：
Zhao Ye
中科院分区：
文献类型：
--
作者：
Mao Yiwen;Tao Rui;Cao Xiaoping;Bao Qin;Wang Dong;Zhao Ye
目的：探讨CCR10在辐射致皮肤损伤中先天淋巴样细胞（ILCs）应答中的作用。材料和方法：CCR10þ/和CCR10/小鼠分别给予5Gy或5Gy单次剂量，x射线总剂量为30Gy，连续6天。照射后3天和10天，从皮肤中分离ILCs，用流式细胞术分析。采用放射性皮炎小鼠模型，观察65Gy辐照后10d的皮肤损伤情况。结果：单次照射后3d， CCR10þ/和CCR10/小鼠皮肤ILCs均降低（p< 0.05）。然而，单次照射10天后，皮肤炎症消失，ilc恢复正常水平。65Gy辐照后，两种基因型的ILCs也均下降，但CCR10/小鼠皮肤ILCs百分比低于CCR10þ/小鼠。免疫组化结果显示，CCR10/小鼠的皮肤炎症比CCR10þ/小鼠更严重。结论：与CCR10þ/小鼠相比，CCR10/小鼠辐照后的ILCs百分比更低，皮肤损伤更大。这些发现表明，皮肤ilc受CCR10的调控，可能是减轻放射性皮炎的潜在靶点。
Objective: To assess the role of CCR10 in innate lymphoid cells (ILCs) response in radiationinduced skin damage. Material and methods: CCR10þ/ and CCR10/ mice were treated with either a single dose of 5 Gy or 5Gy everyday for 6days with a total dose of 30Gy with X-ray. ILCs from the skin were isolated and analyzed by flow cytometry 3 and 10days after irradiation. A mouse model of radiodermatitis was used to assess the skin damage 10days after 65Gy irradiation. Results: Skin ILCs were decreased in both CCR10þ/ and CCR10/ mice 3days after single irradiation (p<.05). However, the skin inflammation disappeared and ILCs returned to normal levels 10days after single irradiation. ILCs of both genotypes were also decreased after 65Gy irradiation, but the percentage of skin ILCs in CCR10/ mice was lower than that in CCR10þ/ mice 10days after irradiation. The immunohistochemistry results showed that CCR10/ mice had more severe skin inflammation than CCR10þ/ mice. Conclusion: CCR10/ mice had lower percentages of ILCs and more skin damage than CCR10þ/ mice after irradiation. These findings indicate that skin ILCs are regulated by CCR10, which might be a potential target for reducing the radio-dermatitis.