A fluorous capping strategy for Fmoc-based automated and manual solid-phase peptide synthesis
A fluorous capping strategy for Fmoc-based automated and manual solid-phase peptide synthesis
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DOI：
10.1002/ejoc.200500958
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发表时间：
2006-02-13
影响因子：
2.8
通讯作者：
Kumar, K
中科院分区：
文献类型：
--
作者：
Montanari, V;Kumar, K
加水：通过使用标准化Fmoc方案用商业自动化合成仪合成的肽可以通过水溶液的简单离心从缺失产物中纯化。缺失产物用氟三价碘鎓盐封端。在合成结束时，将粗肽溶于水中并离心，缺失产物沉淀，仅留下全长肽在溶液中。协议的普遍使用这一战略的报告。(c)Wiley-VCH Verlag GmbH & Co. KGaA，69451魏因海姆，德国，2006.
Just add water: Peptides synthesized by the use of standardized Fmoc protocols with commercial automated synthesizers can be purified from deletion products by simple centrifugation of aqueous solutions. The deletion products are capped with fluorous trivalent iodonium salts. At the end of the synthesis, the crude peptide is dissolved in water and centrifuged, and the deletion products precipitate leaving only the full length peptide in solution. Protocols for generalized use of this strategy are reported. (c) Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2006.