The tryptophan cluster: a hypothetical structure of the DNA-binding domain of the myb protooncogene product.
The tryptophan cluster: a hypothetical structure of the DNA-binding domain of the myb protooncogene product.
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DOI：
10.1016/s0021-9258(17)45472-x
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发表时间：
1990-11
期刊：
影响因子：
--
通讯作者：
C. Kanei-Ishii;A. Sarai;Tetsuya Sawazaki;H. Nakagoshi;Dong-Ning He;Kazuhiro Ogata;Yoshifumi Nishimura-Yoshifumi
中科院分区：
文献类型：
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作者：
C. Kanei-Ishii;A. Sarai;Tetsuya Sawazaki;H. Nakagoshi;Dong-Ning He;Kazuhiro Ogata;Yoshifumi Nishimura-Yoshifumi
c-myb 原癌基因产物 (c-Myb) 的 DNA 结合结构域由 3 个 51-52 个氨基酸的重复序列组成，每个重复序列中有 3 个完全保守的色氨酸。这些色氨酸的定点诱变表明，色氨酸对亲水残基或丙氨酸的任何单个或多个突变都会消除或大大降低序列特异性DNA结合活性，但对疏水氨基酸的突变保留了相当大的活性。拉曼光谱研究表明这些色氨酸埋藏在蛋白质核心中。建议这 3 个色氨酸在每次重复中在疏水核心中形成簇。这种假设的结构被称为“色氨酸簇”，它可能代表了一组 DNA 结合蛋白（包括 myb 和 ets 相关蛋白）的特征。
In the DNA-binding domain of the c-myb protooncogene product (c-Myb) which consists of three repeats of 51-52 amino acids, there are 3 perfectly conserved tryptophans in each repeat. Site-directed mutagenesis of these tryptophans showed that any single or multiple mutations of tryptophan to hydrophilic residues or alanine abolished or greatly reduced the sequence-specific DNA-binding activity, but mutations to hydrophobic amino acids retained considerable activity. Raman spectroscopic study showed that these tryptophans were buried in the protein core. These 3 tryptophans are proposed to form a cluster in the hydrophobic core in each repeat. This hypothetical structure is referred to as the “tryptophan cluster,” and it may represent a characteristic property of a group of DNA-binding proteins including the myb- and ets-related proteins.