Molecular cloning and expression of Chimonanthus praecox farnesyl pyrophosphate synthase gene and its possible involvement in the biosynthesis of floral volatile sesquiterpenoids
Molecular cloning and expression of Chimonanthus praecox farnesyl pyrophosphate synthase gene and its possible involvement in the biosynthesis of floral volatile sesquiterpenoids
复制标题
腊梅法尼基焦磷酸合酶基因的分子克隆和表达及其在花挥发性倍半萜生物合成中的作用
DOI：
10.1016/j.plaphy.2010.08.015
复制
发表时间：
2010-10-01
影响因子：
6.5
通讯作者：
Chen, Longqing
中科院分区：
文献类型：
--
作者：
Xiang, Lin;Zhao, Kaige;Chen, Longqing
法尼基焦磷酸（FPP）合成酶催化异戊烯焦磷酸（IPP）和二甲基烯丙基焦磷酸（DMAPP）合成法尼基焦磷酸（FPP），法尼基焦磷酸（FPP）是倍半萜类化合物如花香挥发物的前体。采用RT-PCR和RACE方法克隆蜡梅FPP合成酶基因。推导的氨基酸序列与植物FPP淀粉酶具有较高的同源性。该基因在大肠杆菌中的表达产生了FPPS活性，其催化FPP作为主要产物的合成。组织特异性和发育分析的CpFPPS的mRNA水平和挥发性倍半萜类化合物的含量在C praecox花揭示FPPS可能发挥调控作用的花挥发性倍半萜类化合物。(C)2010年Elsevier Masson SAS。All rights reserved.
Farnesyl pyrophosphate (FPP) synthase catalyzes the biosynthesis of FPP, which is the precursors of sesquiterpenoids such as floral scent volatiles, from isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP). cDNA encoding wintersweet (Chimonanthus praecox L) FPP synthase was isolated by the RT-PCR and RACE methods. The deduced amino acid sequence showed a high identity to plant FPP synthases. Expression of the gene in Escherichia coli yielded FPPS activity that catalyzed the synthesis of FPP as a main product. Tissue-specific and developmental analyses of the mRNA levels of CpFPPS and volatile sesquiterpenoids levels in C praecox flowers revealed that the FPPS may play a regulatory role in floral volatile sesquiterpenoids of wintersweet. (C) 2010 Elsevier Masson SAS. All rights reserved.