FORMATION AND METABOLISM OF PUNGENT PRINCIPLE OF CAPSICUM FRUITS .14. INVIVO AND INVITRO METABOLISM OF DIHYDROCAPSAICIN, A PUNGENT PRINCIPLE OF HOT PEPPER, IN RATS
FORMATION AND METABOLISM OF PUNGENT PRINCIPLE OF CAPSICUM FRUITS .14. INVIVO AND INVITRO METABOLISM OF DIHYDROCAPSAICIN, A PUNGENT PRINCIPLE OF HOT PEPPER, IN RATS
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DOI：
10.1080/00021369.1985.10866743
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发表时间：
1985-01-01
期刊：
影响因子：
--
通讯作者：
IWAI, K
中科院分区：
文献类型：
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作者：
KAWADA, T;IWAI, K
采用薄层色谱法、高效液相色谱法和气相色谱-质谱联用技术研究了辣椒辣素在大鼠体内和体外的代谢。雄性成年大鼠口服二氢辣椒素（20 mg/kg体重）48小时内，在尿液中鉴定出原型二氢辣椒素及其8种代谢产物;即，游离形式的二氢辣椒素（总剂量的8.7%）、香草胺（4.7%）、香草醛（4.6%）、香草醇（37.6%）和香草酸（19.2%）和/或其葡糖苷酸。尿液中游离和葡糖苷酸代谢产物的比例分别为总剂量的14.5%和60.5%。部分原型二氢辣椒碱（总剂量的10%）在48小时内排泄到粪便中。大鼠肝无细胞提取物催化二氢辣椒素水解为香草胺和8-甲基壬酸。前者进一步原位转化为香草醛。二氢辣椒素水解酶活性在大鼠各器官中均有表达。活性主要位于肝脏。在此基础上，提出了二氢辣椒碱在大鼠体内的代谢途径。
The metabolism in rats of dihydrocapsaicin, a pungent principle of hot pepper, was investigated in vivo and in vitro by TLC, high-performance liquid chromatography and combined gas chromatography-mass spectrometry. Within 48 h of oral administration of dihydrocapsaicin (20 mg/kg body weight) to male adult rats, unchanged dihydrocapsaicin and eight of its metabolites were identified in urine; i.e., dihydrocapsaicin (8.7% of total dose), vanillylamine (4.7%), vanillin (4.6%), vanillyl alcohol (37.6%) and vanillic acid (19.2%) as free forms and or their glucuronides. The proportions of free and glucuronide metabolites in urine were 14.5% and 60.5% of the total dose. Part of the unchanged dihydrocapsaicin (10% of total dose) was excreted into the feces within 48 h. Cell-free extracts of rat liver catalyzed the hydrolysis of dihydrocapsaicin to vanillylamine and 8-methyl nonanoic acid. The former compound was further transformed to vanillin in situ. Dihydrocapsaicin-hydrolyzing enzyme activity was found in various organs of rat. The activity was located mainly in the liver. On the basis of the present data, the metabolic pathway of dihydrocapsaicin in rats was proposed.