IDENTIFICATION OF EPSTEIN-BARR VIRUS-INFECTED CELLS IN TONSILS OF ACUTE INFECTIOUS-MONONUCLEOSIS BY INSITU HYBRIDIZATION
IDENTIFICATION OF EPSTEIN-BARR VIRUS-INFECTED CELLS IN TONSILS OF ACUTE INFECTIOUS-MONONUCLEOSIS BY INSITU HYBRIDIZATION
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DOI：
10.1016/0046-8177(89)90075-0
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发表时间：
1989-08-01
期刊：
影响因子：
3.3
通讯作者：
STEIN, H
中科院分区：
文献类型：
--
作者：
NIEDOBITEK, G;HAMILTONDUTOIT, S;STEIN, H
原位杂交与35S标记的EB病毒（EBV）探针应用于从临床，血清学和形态学证据的急性传染性单核细胞增多症的7例患者的扁桃体石蜡切片。在所有这些病例中，在滤泡间和滤泡周围区的活化淋巴B母细胞中证实了EBV基因组。然而，在任何情况下都不能在上皮细胞中鉴定出EBV。这些结果与目前认为扁桃体上皮在原发性EBV感染中起中心作用的观点不同。
In situ hydbridization with 35S-labeled Epstein-Barr virus (EBV) probes was applied to paraffin sections of tonsils from seven patients with clinical, serologic, and morphologic evidence of acute infectious mononucleosis. EBV genomes were demonstrated in activated lymphoid B blasts in the interfollicular and perifollicular zones in all these cases. However, in no case could EBV be identified in epithelial cells. These results are at variance with the current concept which attributes a central role to the tonsillar epithelium in primary EBV infection.