[Identification and characterization of the outermost layer of Bacillus subtilis spores].
[Identification and characterization of the outermost layer of Bacillus subtilis spores].
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DOI：
10.1248/yakushi.132.919
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
D. Imamura
中科院分区：
文献类型：
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作者：
D. Imamura
革兰氏阳性细菌枯草芽孢杆菌在不适合生长的条件下形成孢子。孢子被包裹在包括皮层和孢子衣的多层壳中，并在恶劣环境中保持长时间的活力。本文综述了近年来对B的外部结构的研究进展。枯草芽孢杆菌孢子是审查在日本的语言。虽然孢子外壳组装涉及至少70种不同蛋白质的沉积，但大多数此类蛋白质的位置尚未通过实验确定。为此，使用荧光显微镜测量蛋白质层和孢子的直径，然后测量蛋白质在B的孢子衣中的位置。枯草芽孢杆菌孢子估计。用此方法测定了16个蛋白质的位置。一种蛋白质被分配给皮层，九种分配给内层，四种分配给外层。此外，两种蛋白质，CgeA和CotZ，被分配到一个以前未确定的最外层。McKenney等人也使用类似的方法确定了最外层;该层被称为“地壳”。免疫荧光显微镜显示，外壳确实是最外层的B。枯草芽孢突变分析表明，所有的基因在cotVWXYZ簇参与孢子壳的合成，CotY和CotZ在壳的形成中发挥关键作用。
The Gram-positive bacterium Bacillus subtilis forms spores when conditions are unsuitable for growth. The spores are encased in a multilayered shell that includes a cortex and a spore coat, and remain viable for long periods in the harsh environment. In the present article, recent progress in our understanding of the outer structure of B. subtilis spores is reviewed in the Japanese language. Although spore coat assembly involves the deposition of at least 70 distinct protein species, the positions of most of such proteins have not been experimentally determined. To this end, the diameters of the protein layers and spores were measured using fluorescence microscopy and then the positions of proteins in the spore coat of B. subtilis spores were estimated. The locations of 16 proteins were determined using this method. One protein was assigned to the cortex, nine to the inner coat, and four to the outer coat. Further, two proteins, CgeA and CotZ, were assigned to a previously unidentified outermost layer. McKenney et al. have also identified the outermost layer using a similar method; the layer was termed the "crust". Immunofluorescence microscopy revealed that the crust is indeed the most external layer of B. subtilis spores. Mutational analysis indicated that all genes in the cotVWXYZ cluster were involved in spore crust synthesis and that CotY and CotZ played critical roles in crust formation.