Automated Cerebrospinal Fluid Cell Counts Using the New Body Fluid Mode of Sysmex UF-1000i
Automated Cerebrospinal Fluid Cell Counts Using the New Body Fluid Mode of Sysmex UF-1000i
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DOI：
10.1002/jcla.21866
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发表时间：
2016-09-01
影响因子：
2.7
通讯作者：
Lippi, Giuseppe
中科院分区：
文献类型：
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作者：
Buoro, Sabrina;Esposito, Sara Apassiti;Lippi, Giuseppe
背景资料：我们评估了Sysmex UF 1000 i（UF 1000 i-BF）上的新体液模块，用于分析脑脊液（CSF）中的白色血细胞（WBC）和红细胞（RBC）。方法：对67例脑脊液标本分别用UF 1000 i-BF和Fuchs-Rosenthal计数箱进行白细胞和红细胞计数。本研究还包括日间精密度、空白限（LoB）、检测限（LoD）、功能灵敏度（定量限，LoQ）、残留和线性评价。还评估了白细胞计数正常和升高（>= 5.0 × 10（6）/L）之间的诊断一致性。结果：UF 1000 i-BF法与人工白细胞计数法在所有脑脊液标本中的一致性均不显著（r = 0.99; y = 1.05x + 0.09）。在WBC < 30 x 10（6）/L的样本中观察到适度高估（r = 0.95; y = 1.21 x-0.15）。观察到RBC计数的良好一致性（r = 0.98; y = 1.15x + 0.55），特别是在RBC >= 18 x 10（6）/L的样本中（r = 0.98; y = 1.01x + 8.90）。日间精密度良好，WBC和RBC的变异系数（CV）均低于7.2%。LoB分别为0.1 × 10（6）WBC/L和1.2 × 10（6）RBC/L，LoD分别为0.7 × 10（6）WBC/L和5.5 × 10（6）RBC/L，LOQ分别为2.4 × 10（6）WBC/L和18.0 × 10（6）RBC/L。线性良好（WBC和RBC均为r = 1.00）。残留可忽略不计。在4.5 × 10（6）WBC/L临界值时获得了极好的诊断一致性（敏感性，100%;特异性，97.4%）。结论：UF 1000 i-BF可提供快速、准确的WBC和RBC计数，且符合CSF细胞的临床相关值。因此，UF 1000 i-BF的使用可以替代常规光学计数，但显示异常WBC计数或异常散点图分布的样本除外，这些样本可能仍需要进行细胞分类计数。(C)2015 Wiley Periodicals，Inc.
Background: We evaluated the new body fluid module on Sysmex UF1000i (UF1000i-BF) for analysis of white blood cell (WBC) and red blood cell (RBC) in cerebrospinal fluid (CSF). Methods: WBC and RBC counting were compared between UF1000i-BF and Fuchs-Rosenthal counting chamber in 67 CSF samples. This study also included the evaluation of between-day precision, limit of blank (LoB), limit of detection (LoD), functional sensitivity (limit of quantitation, LoQ), carryover and linearity. Diagnostic agreement for differentiation between normal and increased WBC counts (>= 5.0 x 10(6)/L) was also assessed. Results: The agreement between UF1000i-BF and manual WBC counts was otpiaml in all CSF samples (r = 0.99; y = 1.05x + 0.09). A modest overestimation was noticed in samples with WBC < 30 x 10(6)/L (r = 0.95; y = 1.21x -0.15). A good agreement was observed for RBC counts (r = 0.98; y = 1.15x + 0.55), particularly in samples with RBC >= 18 x 10(6)/L (r = 0.98; y = 1.01x + 8.90). Between-day precision was good, with coefficient of variations (CVs) lower than 7.2% for both WBC and RBC. The LoBs were 0.1 x 10(6) WBC/L and 1.2 x 10(6) RBC/L, the LoDs were 0.7 x 10(6) WBC/L and 5.5 x 10(6) RBC/L, the LoQswere 2.4x10(6) WBC/L and 18.0 x 10(6) RBC/L, respectively. Linearity was excellent (r = 1.00 for bothWBC and RBC). Carryover was negligible. Excellent diagnostic agreement was obtained at 4.5 x 10(6) WBC/L cut-off (sensitivity, 100%; specificity, 97.4%). Conclusion: The UF1000i-BF provides rapid and accurate WBC and RBC counts in clinically relevant values of CSF cells. The use of UF1000i-BF may hence allow to replace routine optical counting, except for samples displaying abnormal WBC counts or abnormal scattergram distribution, for which differential cell counts may still be required. (C) 2015 Wiley Periodicals, Inc.