EFFECT OF BASIC FIBROBLAST GROWTH-FACTOR AND ALPHA-MELANOCYTIC STIMULATING HORMONE ON NERVE REGENERATION THROUGH A COLLAGEN CHANNEL
EFFECT OF BASIC FIBROBLAST GROWTH-FACTOR AND ALPHA-MELANOCYTIC STIMULATING HORMONE ON NERVE REGENERATION THROUGH A COLLAGEN CHANNEL
复制标题
DOI：
10.1002/micr.1920150312
复制
发表时间：
1994-01-01
期刊：
影响因子：
2.1
通讯作者：
TADIE, M
中科院分区：
文献类型：
--
作者：
LAQUERRIERE, A;PEULVE, P;TADIE, M
通过对大鼠坐骨神经的实验研究，评价胶原引导下的神经再生，并研究α-黑素细胞刺激素(α-MSH)和碱性成纤维细胞生长因子(b-FGF)在促进轴突延长中的作用。横断后，使用胎盘IV型胶原导向器修复超过7 mm间隙的神经。该通道用b-成纤维细胞生长因子溶液或α-MSH溶液填充，或用b-成纤维细胞生长因子结合到导轨中生产。四周后，只有注射或掺入b-成纤维细胞生长因子的组显示出显著的体感诱发电位反应。神经纤维的组织学和定量分析证实，在接受α-MSH溶液或含有b-FGF的通道的组中，神经连续性的存在。这些结果表明，溶液中的α-MSH和掺入IV型胶原通道中的b-成纤维细胞生长因子促进周围神经再生。然而，在4周时，只有b-成纤维细胞生长因子(3 Ng)能恢复功能。(C)1994年Wiley-Liss，Inc.
An experimental study on the rat sciatic nerve was performed to evaluate nerve regeneration through a collagen guide and to study the effects of alpha-melanocytic stimulating hormone (alpha-MSH) and basic fibroblast growth factor (b-FGF) in accelerating axonal elongation. After transection, nerves were repaired over a 7 mm gap using a placental collagen type IV guide. The channel was filled with either a b-FGF solution or an alpha-MSH solution or was produced with b-FGF incorporated into the guide. Four weeks later, only groups in which b-FGF had been injected or incorporated displayed a significant somatosensory evoked potential response. Histological and quantitative analysis of nerve fibres confirmed the existence of nerve continuity in groups receiving an alpha-MSH solution or a channel containing b-FGF. These results demonstrate that alpha-MSH in solution and b-FGF incorporated into a collagen type IV channel enhance peripheral nerve regeneration. However, at 4 weeks, only b-FGF (3 ng) restores functional activity. (C) 1994 Wiley-Liss, Inc.