Isolation and characterization of murine Cds (CDP-diacylglycerol synthase) 1 and 2
Isolation and characterization of murine Cds (CDP-diacylglycerol synthase) 1 and 2
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DOI：
10.1016/j.gene.2005.04.037
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发表时间：
2005-08-15
期刊：
影响因子：
3.5
通讯作者：
Halford, S
中科院分区：
文献类型：
--
作者：
Inglis-Broadgate, SL;Ocaka, L;Halford, S
果蝇的光信号转导是由肌醇磷脂介导的信号转导途径。磷脂酰肌醇4，5-二磷酸(PIP(2))在这一过程中起核心作用，其水平受到严格调控。CDP-二酰甘油合成酶(CDS)是一种光感受器专一性的酶，它催化磷脂酸生成CDP-二酰甘油，是可用于信号转导的PIP2量的关键调节因子。CDS突变体会发生光诱导的视网膜退化。我们在这里报告了编码该酶的两个小鼠基因的分离和特性，即CDS1和CDS2。这些基因编码的蛋白质有73%相同，92%相似，但表现出非常不同的表达模式。CDS1表现出非常有限的表达模式，但在光感受器的内节表达，而CDS2表现出无处不在的表达模式。利用荧光原位杂交技术，将CDs1和CDs2分别定位于染色体5E3和2G1。这些区域与相应的人类基因定位(4q21和20p13)是同步的。表位标记蛋白的瞬时转染实验也表明，两者都与内质网有关。(C)2005 Elsevier B.V.保留所有权利。
Phototransduction in Drosophila is a phosphoinositide-mediated signalling pathway. Phosphatidylinositol 4,5-bisphosphate (PIP(2)) plays a central role in this process, and its levels are tightly regulated. A photoreceptor-specific form of the enzyme CDP-diacylglycerol synthase (CDS), which catalyzes the formation of CDP-diacylglycerol from phosphatidic acid, is a key regulator of the amount Of PIP2 available for signalling. cds mutants develop light-induced retinal degeneration. We report here the isolation and characterization of two murine genes encoding this enzyme, Cds1 and Cds2. The genes encode proteins that are 73% identical and 92% similar but exhibit very different expression patterns. Cds1 shows a very restricted expression pattern but is expressed in the inner segments of the photoreceptors whilst Cds2 shows a ubiquitous pattern of expression. Using fluorescent in situ hybridization we have mapped Cds1 and Cds2 to chromosomes 5E3 and 2G1 respectively. These are regions of synteny with the corresponding human gene localization (4q21 and 20p13). Transient transfection experiments with epitope tagged proteins have also demonstrated that both are associated with the endoplasmic reticulum. (c) 2005 Elsevier B.V. All rights reserved.