Preparation and Inflammasome Activation of Murine Bone Marrow-Derived and Resident Peritoneal Macrophages
Preparation and Inflammasome Activation of Murine Bone Marrow-Derived and Resident Peritoneal Macrophages
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鼠骨髓源性腹膜巨噬细胞的制备和炎症小体激活
DOI：
10.1007/978-1-0716-1971-1_9
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Kaisho T.
中科院分区：
文献类型：
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作者：
Sasaki I;Kaisho T.
霍乱弧菌分泌的霍乱毒素（Cholera toxin，CT）不仅能引起霍乱，而且具有免疫佐剂的作用.巨噬细胞对多种免疫佐剂有反应，是非常异质的，它们的发育和功能取决于它们所在的组织。本实验研究了CT的B亚单位（CT B）对小鼠骨髓源性巨噬细胞（BBM）和常驻腹腔巨噬细胞（rPM）的作用。CTB可协同脂多糖诱导两种巨噬细胞产生白细胞介素-1 β（IL-1β）。然而，IL-1β诱导的潜在分子机制不同。在这里，我们描述的协议，准备和刺激的BMPs和RPM。
Cholera toxin (CT), secreted byVibrio cholerae, not only causes cholera but also functions as an immune adjuvant. Macrophages, which respond to a variety of immune adjuvants, are quite heterogenous and their development and function depend on the tissues where they are localized. We have characterized the effects of the B subunit of CT (CTB) on two types of murine macrophages, that is, bone marrow–derived macrophages (BMMs) and resident peritoneal macrophages (rPMs). CTB could induce production of interleukin-1β (IL-1β) from both macrophages in synergy with lipopolysaccharides. However, underlying molecular mechanisms for IL-1β induction were different. Here, we describe the protocols for preparation and stimulation of BMMs and rPMs.