Methylparaben in Anopheles gambiae s.l. sugar meals increases longevity and malaria oocyst abundance but is not a preferred diet
Methylparaben in Anopheles gambiae s.l. sugar meals increases longevity and malaria oocyst abundance but is not a preferred diet
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DOI：
10.1016/j.jinsphys.2008.11.003
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发表时间：
2009-03-01
影响因子：
2.2
通讯作者：
Wilkins, Elien E.
中科院分区：
文献类型：
--
作者：
Benedict, Mark Q.;Hood-Nowotny, Rebecca C.;Wilkins, Elien E.
将抗微生物和抗真菌化学品对羟基苯甲酸甲酯（甲基-4-羟基苯甲酸酯）添加到冈比亚按蚊和阿拉伯按蚊的成年蔗糖饮食中，并测定其对寿命的影响。在所有情况下，观察到0.2%（w/v）对羟基苯甲酸甲酯添加到每周刷新的膳食中时，寿命显着增加。当每天更新新鲜的糖饮食时，没有观察到由于对羟基苯甲酸甲酯而导致的寿命增加，这表明对羟基苯甲酸甲酯的作用是保持糖饮食的质量。没有观察到除了补充糖或对羟基苯甲酸甲酯的膳食之外提供纯净水的长寿效应。喂食偏好测试进行，以确定是否含有对羟基苯甲酸甲酯的膳食是首选，以及是否，当没有选择，但不太喜欢的饮食，蚊子会消耗更少的糖。在配对试验中使用稳定的碳同位素（13）C，我们表明，含有对羟基苯甲酸甲酯的糖饮食明显避免了A。gambiae而不是A.阿拉伯的。当蚊子没有饮食选择时，观察到对羟基苯甲酸甲酯对糖消耗总量的影响很小。对羟基苯甲酸甲酯对食蟹猴疟原虫B卵囊形成和包囊的影响在正常Agambiae原液和高频率包囊原液中观察到。由于饮食中的对羟基苯甲酸甲酯，观察到正常卵囊和包囊卵囊数量增加近两倍。由于它的长寿效果，我们已经实施了对羟基苯甲酸甲酯使用所有蚊子在我们的控股，并建议它作为一个例行的糖餐补充。(C)2009年由Elsevier Ltd.出版
The antimicrobial and antifungal chemical methylparaben (methyl-4-hydroxybenzoate) was added to the adult sucrose diet of Anopheles gambiae and Anopheles arabiensis, and its effect on longevity was determined. In all cases, significant increases in longevity were observed when 0.2% (w/v) methylparaben was added to meals that were refreshed weekly. When fresh sugar diet was refreshed daily, no increase in longevity was observed due to methylparaben suggesting that the effect of methylparaben is to preserve the quality of the sugar diet. No longevity effect of providing pure water in addition to sugar- or methylparaben-supplemented meals was observed. Feeding preference tests were performed to determine whether meals containing methylparaben were preferred, and whether, when given no choice but the less-preferred diet, mosquitoes would consume less sugar. Using the stable carbon isotope (13)C in paired tests, we show that the sugar diet containing methylparaben was clearly avoided by A. gambiae but not A. arabiensis. Little effect of methylparaben on the total amount of sugar consumed was observed when mosquitoes were given no diet choice. Methylparaben effects on Plasmodium cynomolgi B oocyst formation and encapsulation were observed in a normal Agambiae stock and one which encapsulates at a high frequency. Nearly two-fold increases in the number of both normal and encapsulated oocysts were observed as a result of methylparaben in the diet. Because of its longevity effects, we have implemented methylparaben use for all mosquitoes in our holdings and recommend it as a routine sugar meal supplement. (C) 2009 Published by Elsevier Ltd.