DISTRIBUTION OF CORTICOTROPIN-RELEASING FACTOR-LIKE IMMUNOREACTIVITY IN SQUIRREL-MONKEY (SAIMIRI-SCIUREUS) AMYGDALA
DISTRIBUTION OF CORTICOTROPIN-RELEASING FACTOR-LIKE IMMUNOREACTIVITY IN SQUIRREL-MONKEY (SAIMIRI-SCIUREUS) AMYGDALA
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DOI：
10.1002/cne.903230108
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发表时间：
1992-09-01
影响因子：
2.5
通讯作者：
FOOTE, SL
中科院分区：
文献类型：
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作者：
BASSETT, JL;FOOTE, SL
先前对大鼠脑中促肾上腺皮质激素释放因子（CRF）样免疫反应性的解剖研究已经报道了杏仁核中含有这种肽的神经元元件的突出聚类。crf阳性细胞和纤维的最高浓度在中央核中都很明显，这一观察结果与该肽在自主神经和内分泌调节中的假设作用一致。此外，在其他杏仁核中也发现了低密度的crf阳性体和突起。然而，在任何灵长类动物中，扁桃体中crf样免疫反应性的分布尚未被描述。这样的描述将会引起人们的兴趣，因为在啮齿类动物和灵长类动物中，CRF在其他大脑区域的分布存在实质性差异。本研究使用免疫组织化学方法，使用针对人形式的CRF的多克隆抗血清，确定该肽在未使用秋水仙碱治疗的猴子（samiri sciureus）中的分布。在杏仁核复合体中，基底核和外侧核中可见最多和最集中的crf阳性神经元集合。crf阳性纤维和终末密度最高的是外侧和中央杏仁核。杏仁核周围皮层第1层、外侧嗅束核、皮层前核、后核和内侧核也可见中度致密的crf阳性纤维丛。因此，crf样免疫反应性在猴子和大鼠杏仁核中的分布有很大的不同。由于猴核外核中crf阳性在与新皮层有明显联系的核中最为突出，这些差异可能是灵长类动物大脑皮层发育加快的一个组成部分。
Previous anatomical studies of corticotropin-releasing factor (CRF)-like immunoreactivity in rat brain have reported prominent clustering of neuronal elements containing this peptide within the amygdala. The highest concentrations of both CRF-positive cells and fibers were evident in the central nucleus, an observation consistent with the putative role of this peptide in autonomic and endocrine regulation. In addition, lower densities of CRF-positive somata and processes have been noted in other amygdaloid nuclei. However, the distribution of CRF-like immunoreactivity in the amygdala has not been described for any primate species. Such a description would be of interest since substantial differences in the distribution of CRF in rodent and primate have been reported for other brain regions.The present study uses immunohistochemical methods, with a polyclonal antiserum directed against the human form of CRF, to determine the distribution of this peptide in non-colchicine-treated monkeys (Saimiri sciureus). Within the amygdaloid complex, the most numerous and concentrated collections of CRF-positive neurons were seen in the basal and lateral nuclei. The highest densities of CRF-positive fibers and terminals were seen in the lateral and central amygdaloid nuclei. Moderately dense plexuses of CRF-positive fibers also were seen in layer la of the periamygdaloid cortex, nucleus of the lateral olfactory tract, anterior and posterior cortical nuclei, and the medial nucleus. Thus, the distribution of CRF-like immunoreactivity differs substantially in monkey and rat amygdala. Since CRF-positive perikarya in monkey are most prominent in nuclei with pronounced interconnections with neocortex, these differences may be an integral component of the increased cortical development that characterizes the primate brain.