Sequence analysis of NSP9 gene of 25 PRRSV strains from Guangdong province, subtropical southern China
Sequence analysis of NSP9 gene of 25 PRRSV strains from Guangdong province, subtropical southern China
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南亚热带广东省25株PRRSV NSP9基因序列分析
DOI：
10.1007/s11262-012-0842-6
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发表时间：
2013-02-01
期刊：
影响因子：
1.6
通讯作者：
Zhang, Guihong
中科院分区：
文献类型：
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作者：
Zhao, Mengmeng;Ning, Zhangyong;Zhang, Guihong
为了解猪繁殖与呼吸综合征在广东省养殖场的流行趋势，于2009-2012年从广东省11个区采集了25株猪繁殖与呼吸综合征病毒(PRRSV)。对25株PRRSV的NSP9基因全序列进行扩增、测序，并与已发表的其他毒株的序列进行比较。结果表明，这些序列与JXA1的核苷酸同源性为97.6-99.4%，氨基酸同源性为97.5-99.4%；与VR2332的核苷酸同源性为91.4-93.1%，氨基酸同源性为95.4-92.6%；与中国古典疫苗株CH-1a的核苷酸同源性为95.1-98.4%，氨基酸同源性为95.3-97.0%。系统发育分析表明，这25株毒株均属于北美型，并进一步分为3个亚型。这些基础数据使我们能够评估PRRS在广东省的流行情况以及PRRSV中nsp9基因的变异和进化。
In order to evaluate the trend in the prevalence of porcine reproductive and respiratory syndrome on farms, 25 porcine reproductive and respiratory syndrome virus (PRRSV) strains were collected from 2009 to 2012 from 11 districts in the Guangdong province. The complete gene sequences of NSP9 from the 25 PRRSV strains were amplified, sequenced, and then compared with the published sequences of other strains. The results showed that these sequences shared 97.6–99.4 % of the same nucleotides and 97.5–99.4 % of the same amino acid sequence identities with JXA1, 91.4–93.1 % of the same nucleotides and 95.4–92.6 % of the same amino acid sequence identities with VR2332, and 95.1–98.4 % of the same nucleotides and 95.3–97.0 % of the same amino acid sequences with the classical Chinese vaccine strain Ch-1a. Phylogenetic analyses showed that all of these 25 strains belonged to the North American genotype and were further divided into three sub-genotypes. This basic data allowed us to evaluate the prevalence of PRRS in the Guangdong province and the variation and evolution of the nsp9 gene in PRRSV.