Effect of retinoic acid and 12-O-tetradecanoyl phorbol-13-acetate on the binding of epidermal growth factor to its cellular receptors.
Effect of retinoic acid and 12-O-tetradecanoyl phorbol-13-acetate on the binding of epidermal growth factor to its cellular receptors.
复制标题
视黄酸和 12-O-十四酰佛波醇-13-乙酸酯对表皮生长因子与其细胞受体结合的影响。
DOI：
10.1016/0304-4165(82)90183-0
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
K. Egawa
中科院分区：
文献类型：
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作者：
M. Saito;I. Ueno;K. Egawa
研究了125i标记的表皮生长因子（EGF）与C3H/2K细胞的结合，以及肿瘤促进剂12- o -十四烷醇-13-乙酸酯（TPA）和肿瘤促进剂维甲酸对其结合的影响。结合的Scatchard图分析显示，存在两种对EGF具有不同亲和力的结合位点。视黄酸处理细胞1小时后，两个位点的亲和力均有所提高，但每个细胞的位点数量没有变化。长时间暴露于视黄酸中消除了这种亲和性的提升，并导致两种类型的环己亚胺结合位点的数量增加。TPA通过消除与高亲和力位点的结合来抑制EGF与细胞的结合，而维甲酸在TPA存在下通过增加低亲和力位点的数量来增强EGF与细胞的结合。
Binding of125I-labelled epidermal growth factor (EGF) to C3H/2K cells and the effect of a tumor promotor, 12-O-tetradecanoyl phorbol-13-acetate (TPA) and of a tumor promotor antagonist, retinoic acid, on the binding was studied. Scatchard plot analysis of the binding showed the presence of two type of binding sites with different affinity to EGF. Treatment of the cells with retinoic acid for 1 h resulted in elevation of the affinity of both sites without changing their number per cell. Prolonged exposure to retinoic acid abrogated this elevation of the affinity and caused cycloheximide-sensitive increase of the number of the binding sites of both types. TPA inhibited binding of EGF to the cells by abolishing the binding to the high affinity sites, whereas retinoic acid, in the presence of TPA, enhanced it by increasing the number of the low affinity sites.
影响因子：
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作者：
Diamond, L;O'Brien, T G;Baird, W M
通讯作者：
Baird, W M