GABA(A) RECEPTOR ANTAGONISM IN THE EXTENDED AMYGDALA DECREASES ETHANOL SELF-ADMINISTRATION IN RATS
GABA(A) RECEPTOR ANTAGONISM IN THE EXTENDED AMYGDALA DECREASES ETHANOL SELF-ADMINISTRATION IN RATS
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DOI：
10.1016/0014-2999(95)00314-b
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发表时间：
1995-09-05
影响因子：
5
通讯作者：
KOOB, GF
中科院分区：
文献类型：
--
作者：
HYYTIA, P;KOOB, GF
本实验研究了大鼠杏仁核GABA（A）受体在乙醇消耗调节中的作用。延伸杏仁核的研究区域包括杏仁核的中央核、终纹的床核和杏仁核的壳。在Wistar大鼠中评估了双侧微量注射竞争性GABA（A）受体拮抗剂2-（3-羧丙基）-3-氨基-6-（4-甲氧基苯基）溴化哒嗪（SR 95531）对乙醇消耗量的影响，这些大鼠接受了训练，在30分钟的会话期间使用糖精褪色程序在两个杠杆、自由选择操作任务中对口服乙醇（10% w/v）作出反应。将SR 95531注射到中央杏仁核中，在2和4 ng的剂量下显著降低乙醇反应，而不影响水反应。SR 95531注射到终纹的床核中，在8 ng和16 ng剂量下显著降低了乙醇反应，而仅16 ng剂量在终纹核的壳中产生了显著影响。累积反应模式表明，杏仁核内注射并没有破坏响应的启动。然而，在最初几分钟内，向终纹和髓核的床核注射抑制了最高SR 95531剂量下的乙醇和水反应。这些发现表明，GABA（A）受体在扩展杏仁核可能参与了乙醇奖励的某些方面的调解。
The present experiments examined the role of the extended amygdala GABA(A) receptors in the regulation of ethanol consumption in rats. The areas of the extended amygdala studied included the central nucleus of the amygdala, the bed nucleus of the stria terminalis, and the shell of the nucleus accumbens. The effects of bilateral microinjections of a competitive GABA(A) receptor antagonist, 2-(3-carboxypropyl)-3-amino-6-(4-methoxyphenyl)pyridazinium bromide (SR 95531), on ethanol consumption were assessed in Wistar rats that were trained to respond for oral ethanol (10% w/v) in a two-lever, free-choice operant task during 30-min sessions using a saccharin fading procedure. Injections of SR 95531 into the central amygdaloid nucleus decreased ethanol responding significantly at doses of 2 and 4 ng without affecting water responding. SR 95531 injections into the bed nucleus of the stria terminalis reduced ethanol responding significantly at the 8 ng and 16 ng dose, while only the 16 ng dose produced a significant effect in the shell of the nucleus accumbens. Cumulative response patterns showed that intra-amygdaloid injections did not disrupt the initiation of responding. Injections into the bed nucleus of the stria terminalis and the nucleus accumbens, however, suppressed both ethanol and water responding at the highest SR 95531 doses during the first minutes. These findings suggest that GABA(A) receptors in the extended amygdala may be involved in the mediation of some aspects of ethanol reward.