FLUCTUATION OF SIZE-FRACTIONATED ALKALINE PHOSPHATASE AFTER BLOOM DISAPPEARANCE IN TWO SHALLOW PONDS
FLUCTUATION OF SIZE-FRACTIONATED ALKALINE PHOSPHATASE AFTER BLOOM DISAPPEARANCE IN TWO SHALLOW PONDS
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两个浅水池水华消失后粒度级碱性磷酸酶的变化
DOI：
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发表时间：
2009
影响因子：
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通讯作者：
Cao Xiuyun
中科院分区：
文献类型：
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作者：
Song Chunlei;Zhou Yiyong;Cao Xiuyun
研究了2001年10月27日至2002年4月15日A、B两个浅水池水华消失后，水华消失后水华的大小、分级碱性磷酸酶(APA)活性和动力学参数以及正磷酸盐(o-P)和叶绿素浓度的时间和垂直变化。A池(微囊藻)水华严重，B池水华不严重。O-P和叶绿素的数据表明，磷是主要的限制性营养元素，其垂直通量应视为藻类生长的重要驱动因素。在A池中，藻类碎屑在水华消失后在上覆水中的积累刺激了藻类部分APA的排泄，主要由负责分解碎屑的附着细菌产生，而细菌部分APA更倾向于在地表水中发挥作用。有趣的是，在B池中观察到了完全相反的现象。在季节中，尽管两个池塘的APA在大小上没有明显的差异，但由于藻类碎屑的输入，A池的总APA较早达到峰值，表现出更高的活性和效率(低K-m和高V-max值)。综上所述，碱性磷酸酶的排泄具有较强的催化效率和较高的活性，应作为明年藻类碎屑分解的重要贡献者，进一步促进营养循环，加速藻类的发育。此外，一些抑制剂和表面活性剂被证明是鉴定溶解碱性磷酸酶来源的良好工具。
The temporal and vertical fluctuations of size fractionated alkaline phosphatase activity (APA) and kinetics parameters as well as orthophosphate (o-P) and chlorophyll concentrations were investigated after bloom disappearance in two shallow ponds A and B from 27 October 2001 to 15 April 2002. Pond A (Microcystis) bloomed seriously but pond B did not. The data of o-P and chlorophyll suggested that phosphorus was the principal limiting nutrimental element and its vertical flux should be regarded as an important driving factor for algal growth. In pond A, the accumulation of algae-derived detritus after bloom disappearance in overlying water stimulated excretion of algal fraction APA, mainly produced by attached bacteria responsible for detritus decomposition, whereas bacterial fraction APA preferred to function in surface water. Interestingly, completely contrary phenomena were observed in pond B. In season, even though no obvious difference for size-fractionated APA in both ponds, the total APA in pond A peaked earlier showing higher activity and efficiency (low K-m and high V-max values) as a result of algal-derived detritus input. In summary, it is suggested that the excretion of alkaline phosphatase with strongly catalyzing efficiency and high activity should be taken as important contributor to algal-derived detritus decomposition, further fueling nutrient recycle and accelerating algal development next year. Furthermore, some inhibitors and surfactants were testified to be good tools to identify the origin of dissolved alkaline phosphatase.