1-WAY STIMULATION IN MIXED LEUKOCYTE CULTURES
1-WAY STIMULATION IN MIXED LEUKOCYTE CULTURES
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DOI：
10.1126/science.153.3735.545
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发表时间：
1966-01-01
期刊：
影响因子：
56.9
通讯作者：
VOYNOW, NK
中科院分区：
文献类型：
--
作者：
BACH, FH;VOYNOW, NK
我们开发了一种改进的混合白细胞培养测试方法。由胸苷掺入率确定的对照值非常低，可以评估同源细胞混合物中的低水平刺激。通过用丝裂霉素 C 处理一个个体的细胞来测定单向刺激；处理过的细胞不能做出反应（掺入胸苷），但仍然可以刺激同源的未处理细胞做出反应。
We have developed an improved method for the mixed leukocyte culture test. Control values, as determined by rates of incorporation of thymidine, are very low, allowing evaluation of low levels of stimulation in homologous cell mixtures. One-way stimulation is assayed by treating the cells of one individual with mitomycin C; treated cells cannot respond (incorporate thymidine) but can still stimulate homologous untreated cells to do so.