Multi-wavelength spectrophotometric determination of hydrogen peroxide in water with peroxidase-catalyzed oxidation of ABTS
Multi-wavelength spectrophotometric determination of hydrogen peroxide in water with peroxidase-catalyzed oxidation of ABTS
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DOI：
10.1016/j.chemosphere.2017.11.091
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发表时间：
2018-02-01
期刊：
影响因子：
8.8
通讯作者：
Cheng, Qingfeng
中科院分区：
文献类型：
--
作者：
Cai, Huahua;Liu, Xin;Cheng, Qingfeng
提出了一种新的分光光度法测定水溶液中过氧化氢的方法。该方法基于过氧化物酶（POD）催化的反应，其中2，2 '-连氮基-双（3-乙基苯并噻唑啉-6-磺酸盐）（ABTS）被氧化形成稳定的绿色自由基（ABTS（中心点+））。生成的ABTS（中心点+）可以通过几何计量法确定。所产生的ABTS（中心点+）在415 nm、650 nm、732 nm和820 nm处的吸光度在0-40 μ M范围内与H2 O2浓度呈线性关系。在415 nm、650 nm、732 nm和820 nm处，ABTS法测定H2 O2的灵敏度分别为6.29 × 10（4）M-1 cm（-1）、2.00 × 10（4）M-1 cm（-1）、2.54 × 10（4）M-1 cm（-1）和1.89 × 10（4）M-1 cm（-1）。在pH6.0的POD催化H2 O2体系中，ABTS氧化生成ABTS（中心点+）的速度非常快，ABTS法的测定时间仅为0.5min，计算出POD存在下H2 O2与ABTS的反应化学计量比接近1：2（1：1.92）。还发现所产生的ABTS（中心点+）的残余吸光度在天然沃茨中在30分钟内是稳定的。ABTS法和DPD法均能准确测定雨水中的低浓度H2 O2，但ABTS法只能在732 nm处准确测定含罗丹明B废水中的H2 O2。此外，H2 O2分析后的废液与拟议的ABTS方法是无害的E。杆菌(C)2017爱思唯尔有限公司版权所有
In this study, a new spectrophotometric method was proposed for the measurement of hydrogen peroxide (H2O2) in aqueous solutions. The method was based on the peroxidase (POD)-catalyzed reaction in which 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) was oxidized to form the stable green radical (ABTS(center dot+)). The generated ABTS(center dot+) could be determined spectrophotometrically. The absorbance of the generated ABTS(center dot+) at 415 nm, 650 nm, 732 nm and 820 nm were linear with H2O2 concentrations in the range of 0-40 mu M. The sensitivities of the proposed ABTS method for H2O2 determination at 415 nm, 650 nm, 732 nm and 820 nm were 6.29 k 10(4) M-1 cm(-1), 2.00 x 10(4) M-1 cm(-1), 2.54 x 10(4) M-1 cm(-1) and 1.89 x 10(4) M-1 cm(-1), respectively. The oxidation of ABTS to generate ABTS(center dot+) in POD-catalyzed H2O2 system at pH 6.0 was so fast that the determination time of the ABTS method was as short as 0.5 min. The stoichiometry of the reaction of H2O2 and ABTS in the presence of POD was calculated as nearly 1:2 (1:1.92). The residual absorbance of the generated ABTS(center dot+) was also found to be stable within 30 min in natural waters. Low H2O2 concentration in rainwater could be both measured by the ABTS method and the DPD method with high accuracy, however, H2O2 concentration in wastewater contained rhodamine B could only be accurately measured with the ABTS method at 732 nm. Moreover, waste solutions after H2O2 analysis with the proposed ABTS method were non-hazardous towards E. coli. (C) 2017 Elsevier Ltd. All rights reserved.