BACTERIA ASSOCIATED WITH EARLY-LIFE STAGES OF HALIBUT, HIPPOGLOSSUS-HIPPOGLOSSUS L, INHIBIT GROWTH OF A PATHOGENIC VIBRIO SP
BACTERIA ASSOCIATED WITH EARLY-LIFE STAGES OF HALIBUT, HIPPOGLOSSUS-HIPPOGLOSSUS L, INHIBIT GROWTH OF A PATHOGENIC VIBRIO SP
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DOI：
10.1111/j.1365-2761.1995.tb01263.x
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发表时间：
1995-01-01
影响因子：
2.5
通讯作者：
BERGH, O
中科院分区：
文献类型：
--
作者：
BERGH, O
在饲养的大比目鱼幼虫的第一次饲养和断奶阶段，从其胃肠道中分离出能够抑制致病性弧菌生长的细菌。在卵表面和卵黄囊幼虫胃肠道分离株中均未发现此类细菌。病原菌抑制剂占分离菌总数的比例在0-100%（首次饲喂）和0-66%（断奶）之间。所有的病原体抑制剂都是革兰氏阴性棒，95%是氧化酶和过氧化氢酶阳性的发酵分离株，能够从不同范围的碳水化合物中产生有氧酸。这些分离株具有弧菌/气单胞菌组的特征，但只有19%的分离株对抑菌剂0/129敏感。从卵和卵黄囊幼虫中分离出的细菌主要属于胞噬菌/柔韧性菌/黄杆菌群。在分离物总数中，具有抑制致病性弧菌生长能力的分离物所占比例很高，这表明病原菌抑制可能是大比目鱼早期肠道菌群的重要互惠作用。
Bacteria capable of inhibiting growth of a pathogenic Vibrio sp. were isolated from the gasrointestinal tract of cultured halibut larvae during the first feeding and weaning stages. No such bacteria were found among isolates from the surface of eggs or the gastrointestinal tract of yolk sac larvae. The fraction of pathogen-inhibitors among the total number of isolates ranged between 0-100% (first feeding) and 0-66% (weaning). All pathogen-inhibitors were Gram-negative rods, and 95% were oxidase and catalase positive fermentative isolates, capable of producing acid aerobically from a varying range of carbohydrates. These isolates possessed the characteristics of the Vibrio/Aeromonas-group, but only 19% were sensitive to the vibriostatic agent 0/129. Isolates from eggs and yolk sac larvae were dominanted by bacteria belonging to the Cytophaga/Flexibacter/Flavobacterium-group. The high fraction of isolates with the ability to inhibit growth of the pathogenic Vibrio sp. among the total number of isolates indicates that pathogen inhibition may be an important mutualistic role of the intestinal flora of early life stages of halibut.