Neral (the Alarm Pheromone) Biosynthesis via the Mevalonate Pathway, Evidenced by D -Glucose-1-13C Feeding in Carpoglyphus lactis and 13C Incorporation into Other Opisthonotal Gland Exudates
Neral (the Alarm Pheromone) Biosynthesis via the Mevalonate Pathway, Evidenced by D -Glucose-1-13C Feeding in Carpoglyphus lactis and 13C Incorporation into Other Opisthonotal Gland Exudates
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Neral（警报信息素）通过甲羟戊酸途径进行生物合成，由乳果蝇中的 D-Glucose-1-13C 喂养和 13C 掺入其他角后腺分泌物证明
DOI：
10.1584/jpestics.29.27
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发表时间：
2004
影响因子：
2.4
通讯作者：
Y. Kuwahara
中科院分区：
文献类型：
--
作者：
Atsushi Morita;N. Mori;R. Nishida;N. Hirai;Y. Kuwahara
单萜橙花醛[（Z）-3，7-二甲基-2，6-辛二烯醛]是一种报警信息素，与十三烷和（Z，Z）-6，9-十七碳二烯一起被鉴定为果蚜后肛腺分泌物的主要成分。通过13 C-NMR分析，发现喂食D-葡萄糖-1-13 C 30天的螨的CDCl 3提取物在2、4、6和8-10位具有13 C原子。因此，得出结论，化合物橙花醛是由2- 13 C-乙酰辅酶A通过糖酵解通过甲羟戊酸途径产生的。在喂食D-葡萄糖-1-13 C 7天后，51.6%的橙花醛分子中至少有一个13 C原子。同样，51.8%的十三烷、42.5%的（Z，Z）-6，9-十七碳二烯、39.5%的γ-螨醛和33.4%的甲酸橙花酯也被标记，而角鲨烯未被标记，表明其来源于培养基。
The monoterpene neral [(Z)-3,7-dimethyl-2,6-octadienal], an alarm pheromone, has been identified as a major component of the opisthonotal gland exudates of Carpoglyphus lactis, together with tridecane and (Z,Z)-6,9-heptadecadiene. The CDCl3 extract of mites fed D-glucose-1-13C for 30 days was found to have 13C atoms at positions 2, 4, 6 and 8–10 by 13C-NMR analysis. The compound neral was, therefore, concluded to be produced via the mevalonate pathway from 2-13C-acetyl-CoA by glycolysis. After seven days of feeding on D-glucose-1-13C, at least one 13C atom was incorporated in 51.6% of neral molecules. Likewise, 51.8% of tridecane, 42.5% of (Z,Z)-6,9-heptadecadiene, 39.5% of γ-acaridial and 33.4% of neryl formate, were also labeled, while squalene was not labeled, indicative of its origin, the culture medium.