Defining synthetic surfaces for human pluripotent stem cell culture.
Defining synthetic surfaces for human pluripotent stem cell culture.
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DOI：
10.1186/2045-9769-2-7
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发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Laslett, Andrew L
中科院分区：
文献类型：
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作者：
Lambshead, Jack W;Meagher, Laurence;Laslett, Andrew L
人类多能干细胞（hPSC）能够无限期地自我更新并分化为所有成体细胞类型。因此，hPSC显示出应用于药物筛选、疾病建模和细胞治疗的潜力。为了满足这种潜力，必须开发一致的、限定的、可扩展的、不含动物产品的培养条件，并且促进hPSC的稳定自我更新。最近有报道称，几种培养表面符合这些标准中的许多标准，尽管由于验证、可靠性和费用问题，这些标准中没有一个被干细胞界广泛实施。大多数hPSC培养表面衍生自细胞外基质蛋白（ECM巧及其细胞粘附分子（CAM）结合基序。阐明CAM介导的细胞-表面相互作用对于体外维持多能性是必不可少的，这将促进hPSC培养表面的优化。报告表明，hPSC培养物可以通过某些CAM亚型的细胞表面相互作用来支持，但不能通过其他亚型来支持。本文总结了最近的报告，确定表面的hPSC文化，并侧重于CAM和ECM参与。
Human pluripotent stem cells (hPSCs) are able to self-renew indefinitely and to differentiate into all adult cell types. hPSCs therefore show potential for application to drug screening, disease modelling and cellular therapies. In order to meet this potential, culture conditions must be developed that are consistent, defined, scalable, free of animal products and that facilitate stable self-renewal of hPSCs. Several culture surfaces have recently been reported to meet many of these criteria although none of them have been widely implemented by the stem cell community due to issues with validation, reliability and expense. Most hPSC culture surfaces have been derived from extracellular matrix proteins (ECMPs) and their cell adhesion molecule (CAM) binding motifs. Elucidating the CAM-mediated cell-surface interactions that are essential for the in vitro maintenance of pluripotency will facilitate the optimisation of hPSC culture surfaces. Reports indicate that hPSC cultures can be supported by cell-surface interactions through certain CAM subtypes but not by others. This review summarises the recent reports of defined surfaces for hPSC culture and focuses on the CAMs and ECMPs involved.