Irradiation of Living Cells with Single Ions at the Ion Microprobe SNAKE
Irradiation of Living Cells with Single Ions at the Ion Microprobe SNAKE
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DOI：
10.12693/aphyspola.109.273
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发表时间：
2006-03
影响因子：
0.7
通讯作者：
A. Hauptner;T. Cremer;M. Deutsch;S. Dietzel;G. A. Drexler;C. Greubel;V. Hable;R. Krücken;R. Löwe;H. Strickfaden;G. Dollinger;A. Friedl
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文献类型：
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作者：
A. Hauptner;T. Cremer;M. Deutsch;S. Dietzel;G. A. Drexler;C. Greubel;V. Hable;R. Krücken;R. Löwe;H. Strickfaden;G. Dollinger;A. Friedl
离子微探针SNAKE的辐照装置用于用单个高能离子辐照活细胞。分别为0.55 μm和0.40 μm的辐照精度允许辐照细胞核的亚结构。通过选择离子的原子序数和能量，可以以超过三个数量级可调节的损伤密度进行辐照。免疫荧光检测技术显示参与DNA双链断裂修复的蛋白质的分布。在第一个实验之一中，检查了活HeLa细胞中辐射诱导病灶的出现动力学。在其他实验中，发现了一种新的效应，涉及在同一细胞核内不同时间点进行的辐照事件之间的相互作用。
The irradiation setup at the ion microprobe SNAKE is used to irradiate living cells with single energetic ions. The irradiation accuracy of 0.55 μm and respectively 0.40 μm allows to irradiate substructures of the cell nucleus. By the choice of ion atomic number and energy the irradiation can be performed with a damage density adjustable over more than three orders of magnitude. Immunofluorescence detection techniques show the distribution of proteins involved in the repair of DNA double-strand breaks. In one of the first experiments the kinetics of appearance of irradiation-induced foci in living HeLa cells was examined. In other experiments a new effect was detected which concerned the interaction between irradiation events performed at different time points within the same cell nucleus.