UVB Generates Microvesicle Particle Release in Part Due to Platelet-activating Factor Signaling.
UVB Generates Microvesicle Particle Release in Part Due to Platelet-activating Factor Signaling.
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DOI：
10.1111/php.12577
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发表时间：
2016-05
影响因子：
3.3
通讯作者：
Travers JB
中科院分区：
文献类型：
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作者：
Bihl JC;Rapp CM;Chen Y;Travers JB
由紫外线B（UVB）产生的脂质介质血小板活化因子（PAF）和氧化甘油磷酸胆碱PAF激动剂已被证明在UVB介导的过程中发挥关键作用，从急性炎症到延迟的全身免疫抑制。最近的研究提供了证据表明，微泡颗粒（MVP）从细胞中释放的各种信号，包括压力。重要的是，这些小的膜片段可以通过传递生物活性分子与各种细胞类型相互作用。本研究旨在测试UVB辐射是否能引起上皮细胞释放MVP，以及PAF受体（PAF-R）信号在这一过程中的潜在作用。我们证明，UVB照射的人角质形成细胞衍生的细胞系HaCaT导致MVP的释放。类似地，用PAF-R激动剂氨甲酰基PAF处理HaCaT细胞也产生等量的MVP释放。值得注意的是，用抗氧化剂预处理HaCaT细胞可以阻断UVB释放MVP，但不能阻断PAF-R激动剂N-甲基氨基甲酰基PAF（CPAF）释放MVP。重要的是，UVB照射PAF-R阴性人上皮细胞系KB和功能性PAF-R转导的KB仅在PAF-R阳性细胞中导致MVP释放。这些研究表明，UVB可以在体外产生MVP，PAF-R信号在这个过程中显得很重要。
The lipid mediator platelet-activating factor (PAF) and oxidized glycerophosphocholine PAF agonists produced by ultraviolet B (UVB) have been demonstrated to play a pivotal role in UVB-mediated processes, from acute inflammation to delayed systemic immunosuppression. Recent studies have provided evidence that microvesicle particles (MVPs) are released from cells in response to various signals including stressors. Importantly, these small membrane fragments can interact with various cell types by delivering bioactive molecules. The present studies were designed to test if UVB radiation can generate MVP release from epithelial cells, and the potential role of PAF receptor (PAF-R) signaling in this process. We demonstrate that UVB irradiation of the human keratinocyte-derived cell line HaCaT resulted in the release of MVPs. Similarly, treatment of HaCaT cells with the PAF-R agonist carbamoyl PAF also generated equivalent amounts of MVP release. Of note, pretreatment of HaCaT cells with antioxidants blocked MVP release from UVB but not PAF-R agonist N-methyl carbamyl PAF (CPAF). Importantly, UVB irradiation of the PAF-R-negative human epithelial cell line KB and KB transduced with functional PAF-Rs resulted in MVP release only in PAF-R-positive cells. These studies demonstrate that UVB can generate MVPs in vitro and that PAF-R signaling appears important in this process.