An ultrastructural study of the influence of acidity of self-etching primers and smear layer thickness on bonding to intact dentin.
An ultrastructural study of the influence of acidity of self-etching primers and smear layer thickness on bonding to intact dentin.
复制标题
DOI：
--
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
F. Tay;H. Sano;R. Carvalho;E. Pashley;D. Pashley
中科院分区：
文献类型：
--
作者：
F. Tay;H. Sano;R. Carvalho;E. Pashley;D. Pashley
本研究的目的是（1）确定使用几种具有不同pH值的自酸蚀底漆系统对完整牙本质的脱矿深度;（2）评价Clearfil SE Bond中完整牙本质的杂交是否会受到涂抹层厚度变化的影响。材料与方法牙本质盘是从人类第三磨牙中提取的中冠牙本质中产生的。将三种自酸蚀底漆系统（Clearfil Liner Bond II、Liner Bond 2 V和SE Bond）分别应用于这些盘，以评价自酸蚀系统穿透玷污层进入完整牙本质的深度。使用“无蚀刻”技术用All-Bond 2处理的牙本质用作对照组。在研究的第二部分中，制作了具有不同玷污层厚度的牙本质盘。冷冻断裂对照组无玷污层。用60-、180-或600粒度的SiC纸研磨实验牙齿，并使用SE Bond粘合。将牙本质盘粘接在一起，并用透射电镜进行观察。结果All-Bond 2没有蚀刻超出涂抹层。三种自酸蚀底漆蚀刻超出涂抹层，以在完整牙本质内形成真正的混合层。使用Liner Bond 2时该层最厚（约1.2至1.4微米），但使用Liner Bond 2 V和SE Bond时该层非常薄（0.5微米）。将SEBond应用于不同表面粗糙度的牙本质，产生不同厚度的杂交玷污层。然而，四个组的底层真杂交体的厚度保持一致（约0.4至0.5微米）。结论：自酸蚀底漆可形成薄的混合层，其中包含涂抹层。怀疑厚的玷污层可能会干扰自酸蚀底漆扩散到下面的完整牙本质中，这一点没有得到证实。
PURPOSE The objectives of this study were (1) to determine the depth of demineralization into intact dentin using several self-etching primer systems with different pH values, and (2) to evaluate whether hybridization of intact dentin in Clearfil SE Bond may be affected by variation in the thickness of the smear layers. MATERIALS AND METHODS Dentin disks were created from mid-coronal dentin in extracted, human third molars. Three self-etching primer systems (Clearfil Liner Bond II, Liner Bond 2V, and SE Bond) were applied separately to these disks to evaluate how deep self-etching systems penetrate through smear layers into intact dentin. Dentin treated with All-Bond 2 using the "no-etch" technique was used as a control group. In the second part of the study, dentin disks with different smear-layer thicknesses were produced. The cryofractured control group was devoid of a smear layer. The experimental teeth were ground with 60-, 180-, or 600-grit SiC paper and bonded using SE Bond. Dentin disks were bonded together and examined with TEM. RESULTS All-Bond 2 did not etch beyond the smear layer. The three self-etching primers etched beyond the smear layer to form true hybrid layers within intact dentin. This layer was thickest with Liner Bond 2 (ca 1.2 to 1.4 microns), but very thin (0.5 micron) using both Liner Bond 2V and SE Bond. Application of SE Bond to dentin of different surface roughness produced hybridized smear layers of variable thickness. However, the thickness of the underlying true hybrid remained consistent for the four groups (ca 0.4 to 0.5 micron). CONCLUSION Self-etching primers create thin hybrid layers that incorporate the smear layer. The suspicion that thick smear layers may interfere with the diffusion of self-etching primers into the underlying intact dentin was not confirmed.