Fluctuations in rat testicular interstitial oxygen tensions are linked to testicular vasomotion: Persistence after repair of torsion
Fluctuations in rat testicular interstitial oxygen tensions are linked to testicular vasomotion: Persistence after repair of torsion
复制标题
DOI：
10.1095/biolreprod63.5.1383
复制
发表时间：
2000-11-01
影响因子：
3.6
通讯作者：
Turner, TT
中科院分区：
文献类型：
--
作者：
Lysiak, JJ;Nguyen, QAT;Turner, TT
众所周知，睾丸微血管血流表现出血管运动，这已被证明是显着改变，在短期内睾丸扭转修复后。这种血管运动的丧失可能最终导致大鼠睾丸扭转后精子发生的丧失。在本研究中，睾丸血管舒缩和间质氧张力同时测量之前，期间，并在不同的时间点后，睾丸扭转修复大鼠。旋转睾丸720 ° 1h，造成睾丸扭转。激光多普勒血流仪和氧电极被用来同时测量血管运动和间质氧张力（PO 2），分别。扭转前对照睾丸的平均血流量为16.3 +/- 1.3灌注单位（PU），显示血管运动，循环频率为12 +/- 0.2循环/分钟，平均振幅为4.2 +/- 0.3 PU。平均睾丸间质PO 2为12.5 +/- 2.6 mm Hg，显示出11.9 +/- 0.4个周期/分钟的周期性变化，平均振幅为2.8 +/- 0.8 mm Hg。在扭转期间，平均血流量和间质PO 2均下降至约为零。复位后，平均微血管血流量和平均间质PO 2值在30 min内恢复到与扭转前值无显著差异的值;然而，即使在24 h后，血管舒缩和PO 2循环也没有恢复。扭转修复后7天，血管舒缩和PO 2循环恢复正常。这些结果首次证明了睾丸血管运动和间质PO 2循环之间的相关性，并且这种相关性在睾丸扭转修复后仍然存在。
Testicular microvascular blood flow is known to exhibit vasomotion, which has been shown to be significantly altered in the short term following the repair of testicular torsion. This loss of vasomotion may ultimately be responsible for the loss of spermatogenesis observed after testicular torsion in rats. In the present study, testicular vasomotion and interstitial oxygen tensions were simultaneously measured prior to, during, and at various time points after repair of testicular torsion in the rat. Testicular torsion was induced by a 720 degrees rotation of the testis for 1 h. Laser-Doppler flowmetry and an oxygen electrode were used to simultaneously measure vasomotion and interstitial oxygen tensions (PO2), respectively. Pretorsion control testes had a mean blood flow of 16.3 +/- 1.3 perfusion units (PU) and displayed vasomotion with a cycle frequency of 12 +/- 0.2 cycles per minute and a mean amplitude of 4.2 +/- 0.3 PU. Mean testicular interstitial PO2 was 12.5 +/- 2.6 mm Hg, which displayed a cyclical variation of 11.9 +/- 0.4 cycles per minute with a mean amplitude of 2.8 +/- 0.8 mm Hg. During the torsion period, both mean blood flow and interstitial PO2 decreased to approximately zero. Upon detorsion, mean microvascular blood flow and mean interstitial PO2 values returned to values that were not significantly different from pretorsion values within 30 min; however, vasomotion and PO2 cycling did not return, even after 24 h. It was 7 days after the repair of torsion before a regular pattern of vasomotion and PO2 cycling returned. These results demonstrate for the first time a correlation between testicular vasomotion and interstitial PO2 cycling, and this correlation persists after the repair of testicular torsion.