Changes in plasma ghrelin levels, gastric ghrelin production, and body weight after Helicobacter pylori cure
Changes in plasma ghrelin levels, gastric ghrelin production, and body weight after Helicobacter pylori cure
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DOI：
10.1007/s00535-006-1880-4
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发表时间：
2006-10-01
影响因子：
6.3
通讯作者：
Sugano, Kentaro
中科院分区：
文献类型：
--
作者：
Osawa, Hiroyuki;Kita, Hiroto;Sugano, Kentaro
背景Ghrelin是一种主要由胃粘膜产生和分泌的体重调节肽。幽门螺杆菌感染损害胃生长素释放肽的产生，导致血浆生长素释放肽浓度降低。然而，H.幽门螺杆菌根除对血浆ghrelin水平的影响及其与H.幽门螺杆菌的治愈仍不确定。我们研究了血浆ghrelin水平与胃ghrelin产生和H前后体重变化的关系。根除幽门方法.在134个连续的个体中，在成功的H之前和之后12周，测定了血浆ghrelin浓度、胃ghrelin表达和体重。根除幽门胃ghrelin表达通过实时逆转录聚合酶链反应和免疫组化分别测定胃粘膜活检标本中ghrelin产生细胞的mRNA表达水平和数量来评估。结果H后50例患者血浆ghrelin浓度升高，84例患者血浆ghrelin浓度降低。根除幽门H. pylori治疗后，胃前促生长素原mRNA表达增加近4倍（P < 0.0001），而ghrelin阳性细胞数增加或无变化。相反，血浆ghrelin在H. pylori治愈率与体重变化（P < 0.0001）和初始血浆ghrelin水平（P < 0.0001）呈负相关。结论. H前后血浆ghrelin浓度的变化。幽门螺杆菌治愈与体重变化和初始血浆ghrelin水平呈负相关，但与日本患者的胃ghrelin产生无关。
Background. Ghrelin is a body weight-regulating peptide produced and secreted primarily by the gastric mucosa. Helicobacter pylori infection impairs gastric ghrelin production, leading to a lower plasma ghrelin concentration. However, the effect of H. pylori eradication on plasma ghrelin levels and its relation to body weight change after H. pylori cure are still uncertain. We examined the association of plasma ghrelin levels with gastric ghrelin production and body weight change before and after H. pylori eradication. Methods. Plasma ghrelin concentrations, gastric ghrelin expression, and body weight were determined in a total of 134 consecutive individuals before and 12 weeks after successful H. pylori eradication. Gastric ghrelin expression was evaluated by determining mRNA expression levels and the number of ghrelin-producing cells in gastric mucosa biopsy specimens by real-time reverse transcriptase-polymerase chain reaction and immunohistochemistry, respectively. Results. Plasma ghrelin concentration increased in 50 patients and decreased in 84 patients after H. pylori eradication. After H. pylori cure, however, gastric preproghrelin mRNA expression was increased nearly fourfold (P < 0.0001), and the number of ghrelin-positive cells was increased or unchanged. In contrast, plasma ghrelin changes after H. pylori cure were inversely correlated with both body weight change (P < 0.0001) and initial plasma ghrelin levels (P < 0.0001). Conclusions. Changes in plasma ghrelin concentrations before and after H. pylori cure were inversely correlated with body weight change and initial plasma ghrelin levels but not with gastric ghrelin production in Japanese patients.