Specific membrane receptors for atrial natriuretic factor in renal and vascular tissues.
Specific membrane receptors for atrial natriuretic factor in renal and vascular tissues.
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肾和血管组织中心房钠尿因子的特异性膜受体。
DOI：
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发表时间：
1984
影响因子：
11.1
通讯作者：
E. H. Blaine
中科院分区：
文献类型：
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作者：
M. Napier;R. Vandlen;G. Albers;R. Nutt;S. Brady;T. Lyle;R. Winquist;E. Faison;L. A. Heinel;E. H. Blaine
兔主动脉膜、兔肾皮质膜和大鼠肾皮质膜对心钠素(ANF)具有高亲和力(Kd=10(-10)M)特异性结合部位。在已建立的猪肾细胞系LLC-PK1中也存在类似的高亲和力位点。分离研究结果表明，这些受体定位于这些组织的质膜。结合是时间依赖的和可饱和的。人工合成的心钠素及其类似物对主动脉膜的亲和力与加入5-羟色胺所致的兔主动脉环松弛所需的半最大浓度之间有很好的定量相关性。此外，受体在肾膜上的结合亲和力与大鼠体内排钠所需的浓度一致。生物上没有活性的合成ANF片段和其他多肽激素，如血管紧张素II和加压素，并不显著抑制结合。这些数据表明，血管和肾脏组织中的ANF受体负责调节这种多肽在这些靶组织中的生理作用。
Membranes from rabbit aorta and from rabbit and rat kidney cortex possess high-affinity (Kd = 10(-10) M) specific binding sites for atrial natriuretic factor (ANF). Similar high-affinity sites are present in an established cell line from pig kidney, LLC-PK1. Results of fractionation studies indicate that the receptors are localized in the plasma membrane of these tissues. The binding is time-dependent and saturable. An excellent quantitative correlation was found between the affinity of synthetic ANF and analogs of intermediate activity to aorta membranes and the half-maximal concentration needed for relaxation of rabbit aorta rings contracted by addition of serotonin. Furthermore, the binding affinity of the receptor in kidney membranes is consistent with the concentration required for in vivo natriuresis in the rat. Biologically inactive synthetic ANF fragments and other peptide hormones such as angiotensin II and vasopressin do not significantly inhibit binding. These data suggest that the receptors for ANF in vascular and renal tissues are responsible for mediating the physiological actions of this peptide in these target tissues.