GPR30 estrogen receptor expression in the growth plate declines as puberty progresses
GPR30 estrogen receptor expression in the growth plate declines as puberty progresses
复制标题
DOI：
10.1210/jc.2007-0814
复制
发表时间：
2007-12-01
影响因子：
5.8
通讯作者：
Savendahl, Lars
中科院分区：
文献类型：
--
作者：
Chagin, Andrei S.;Savendahl, Lars
目的：我们的目的是研究GPR 30是否在骨骺生长板中表达及其作为青春期growth.Background的调节剂的潜在作用：雌激素在骨骼成熟和纵向骨生长的调节中起着至关重要的作用。我们以前已经表明，雌激素受体（ER）α和β在人类骨骺生长板中表达。最近，被称为GPR 30的膜结合ER被发现，但这种受体在纵向骨生长的调节中所发挥的作用尚不清楚。患者/方法：从14名男孩和7名女孩的胫骨生长板进行骨骺手术逮捕纵向骨生长的活检收集。患者处于青春期的不同阶段，腿长不等或身材极高。结果：GPR 30在肥大软骨细胞中表达最高，在静止区也有GPR 30表达阳性的细胞。相反，在增殖区没有发现免疫反应。在青春期的进展有一个明显的下降，GPR 30的表达水平在男孩和girls.Conclusions：新的ER GPR 30在人类生长板中表达，并在青春期进展的表达水平下降。虽然GPR 30表达和年龄之间的关系可能是所观察到的GPR 30水平青春期下降的基础，我们的观察表明，这种受体可能参与青春期纵向骨生长的调节。
Objective: Our objective was to study whether GPR30 is expressed in the epiphyseal growth plate and its potential role as a modulator of pubertal growth.Background: Estrogens play a crucial role in the regulation of skeletal maturation and longitudinal bone growth. We have previously shown that both estrogen receptors (ERs) alpha and beta are expressed in the human epiphyseal growth plate. Recently, a membrane-bound ER referred to as GPR30 was discovered, but the role played by this receptor in the regulation of longitudinal bone growth is not yet known.Patients/Methods: Biopsies were collected from the tibial growth plates of 14 boys and seven girls that underwent epiphyseal surgery to arrest longitudinal bone growth. The patients were in different stages of puberty and suffered from inequality in leg length or extreme tall stature. Paraffin-embedded sections of the growth plates were used to detect expression of the GPR30 protein.Results: The highest level of GPR30 expression was observed in hypertrophic chondrocytes, although cells with positive immunostaining were also detected in the resting zone. In contrast, no immunoreactivity was found in the proliferative zone. During pubertal progression there was a clear decline in the level of GPR30 expression in both boys and girls.Conclusions: The novel ER GPR30 is expressed in the human growth plate, and the level of expression declines during pubertal progression. Although a relationship between GPR30 expression and age may underlie the observed pubertal decline in the GPR30 level, our observations suggest that this receptor could be involved in the modulation of longitudinal bone growth during puberty.