MS-TAFI: A Tool for the Analysis of Fragment Ions Generated from Intact Proteins
MS-TAFI: A Tool for the Analysis of Fragment Ions Generated from Intact Proteins
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DOI：
10.1021/acs.jproteome.2c00594
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发表时间：
2022-12-14
影响因子：
4.4
通讯作者：
Brodbelt，Jennifer S.
中科院分区：
文献类型：
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作者：
Juetten，Kyle J.;Brodbelt，Jennifer S.
完整蛋白质的串联质谱（MS/MS）光谱很难解释，因为片段离子类型和丰度的变化。这些信息对于最大限度地利用自上而下的蛋白质和蛋白质复合物质谱法获得的信息至关重要。MS- tafi（质谱分析工具片段离子）是一个免费的基于python的程序，它提供了一种简化的方法来数据分析和可视化完整蛋白质的反卷积MS/MS数据。该应用程序还包含用于原生质谱实验的工具，具有搜索保留配体（全息离子）的片段离子的能力，以及从193nm紫外光解数据中获得的电荷位点的可视化位置。MS-TAFI的源代码和完整的应用程序可在https://github.com/kylejuetten下载。
Tandem mass spectrometry (MS/MS) spectra of intact proteins can be difficult to interpret owing to the variety of fragment ion types and abundances. This information is crucial for maximizing the information derived from top-down mass spectrometry of proteins and protein complexes. MS-TAFI (Mass Spectrometry Tool for the Analysis of Fragment Ions) is a free Python-based program which offers a streamlined approach to the data analysis and visualization of deconvoluted MS/MS data of intact proteins. The application also contains tools for native mass spectrometry experiments with the ability to search for fragment ions that retain ligands (holo ions) as well as visualize the location of charge sites obtained from 193 nm ultraviolet photodissociation data. The source code and complete application for MS-TAFI is available for download at https://github.com/kylejuetten.