A Dendritic Golgi Satellite between ERGIC and Retromer.
A Dendritic Golgi Satellite between ERGIC and Retromer.
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DOI：
10.1016/j.celrep.2015.12.024
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发表时间：
2016-01
期刊：
影响因子：
8.8
通讯作者：
M. Mikhaylova;S. Bera;O. Kobler;R. Frischknecht;M. Kreutz
中科院分区：
文献类型：
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作者：
M. Mikhaylova;S. Bera;O. Kobler;R. Frischknecht;M. Kreutz
跨膜蛋白的局部合成是神经元可塑性中树突微结构域功能特化的基础。目前尚不清楚这些蛋白质在局部合成后是否能够进入分泌途径的完整机制。在这项研究中，我们描述了一种名为 pGolt 的探针，它可以可视化高尔基体相关的细胞器，从而在神经元中进行实时成像。我们表明，pGolt 标记了一个广泛的微分泌高尔基卫星（GS）系统，与高尔基前哨相反，该系统存在于所有锥体神经元的基底和顶端树突中。 GS 系统包含糖基化机制，位于 ERGIC 和逆转录体之间。突触活动抑制 ERGIC、GS 和逆转录酶彼此靠近的横向运动，从而限制分泌物质的处理。一些突触跨膜蛋白穿过并循环回到 GS 系统。因此，所有神经元树突中 ER-ERGIC-GS-retromer 微分泌系统的存在使得能够自主局部控制跨膜蛋白的合成和加工。
The local synthesis of transmembrane proteins underlies functional specialization of dendritic microdomains in neuronal plasticity. It is unclear whether these proteins have access to the complete machinery of the secretory pathway following local synthesis. In this study, we describe a probe called pGolt that allows visualization of Golgi-related organelles for live imaging in neurons. We show that pGolt labels a widespread microsecretory Golgi satellite (GS) system that is, in contrast to Golgi outposts, present throughout basal and apical dendrites of all pyramidal neurons. The GS system contains glycosylation machinery and is localized between ERGIC and retromer. Synaptic activity restrains lateral movement of ERGIC, GS, and retromer close to one another, allowing confined processing of secretory cargo. Several synaptic transmembrane proteins pass through and recycle back to the GS system. Thus, the presence of an ER-ERGIC-GS-retromer microsecretory system in all neuronal dendrites enables autonomous local control of transmembrane protein synthesis and processing.