Identification of acid-sensing ion channels in bone
Identification of acid-sensing ion channels in bone
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DOI：
10.1016/j.bbrc.2005.09.054
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发表时间：
2005-11-11
影响因子：
3.1
通讯作者：
van Leeuwen, JPTM
中科院分区：
文献类型：
--
作者：
Jahr, H;van Driel, M;van Leeuwen, JPTM
骨平衡血清pH值的变化和破骨细胞和成骨细胞的pH值的微妙变化进行调节。目前的研究的目的是确定骨中的分子，可以感知pH值。有趣的候选人是酸敏感离子通道（ASIC）。在骨中，ASIC 2和ASIC 3是最丰富的，而在软骨细胞中，它是ASIC 1。分离的人单核细胞表达ASIC 1，-2和-3，其在诱导破骨细胞分化后持续存在，尽管水平较低。在人成骨细胞中，显示了ASIC 1、ASIC 2和ASIC 3 mRNA。蛋白质印迹和免疫染色证实了这一点。ASIC 4的表达量一直很低。这是我们第一次在人类骨骼中展示ASIC，提供了一种感知和响应细胞外pH差异的方法。All rights reserved.
Bone balances serum pH variations and both osteoclasts and osteoblasts are regulated by subtle changes in pH. The aim of the current study was to identify molecules in bone that can sense pH. Interesting candidates are the acid-sensing ion channels (ASICs). In bone, ASIC2 and ASIC3 were most abundant, while in chondrocytes it was ASIC1. Isolated human monocytes expressed ASIC1, -2, and -3, which persisted after induction to osteoclast differentiation, albeit to a lower level. In human osteoblasts ASIC1, ASIC2, and ASIC3 mRNAs were shown. Western blot and immunostaining confirmed this at protein level. ASIC4 expression was always very low abundant. For the first time, we demonstrated ASICs in human skeleton, providing a means to sense and respond to differences in extracellular pH. (c) 2005 Elsevier Inc. All rights reserved.