2020 ESC Guidelines for the diagnosis and management of atrial fibrillation developed in collaboration with the European Association for Cardio-Thoracic Surgery (EACTS)
2020 ESC Guidelines for the diagnosis and management of atrial fibrillation developed in collaboration with the European Association for Cardio-Thoracic Surgery (EACTS)
复制标题
DOI：
10.1093/eurheartj/ehaa612
复制
发表时间：
2021-02-01
影响因子：
39.3
通讯作者：
Watkins, Caroline L.
中科院分区：
文献类型：
--
作者：
Hindricks, Gerhard;Potpara, Tatjana;Watkins, Caroline L.
MicroRNAs(MiRNAs)是一种转录后抑制mRNA活性的小分子调控分子。许多不同生物体中的miRNAs已被克隆，但仍有许多未知的miRNAs有待鉴定。在这里，我们描述了从大鼠海马区克隆六个新的miRNAs。其中4个在大鼠miRBase中没有发现，但与人和/或鼠的同源物完全相同，因此命名为RNO-miR-92b、RNO-miR-146b、RNO-let-7g和RNO-miR-551b。另外两个来自已知的miRNA前体RNO-miR-330和RNO-miR-384的另一个臂，在所有生物的miRBase中没有发现。它们被命名为RNO-miR-330*和RNO-miR-384*。RNA拖尾和引物延伸实时逆转录聚合酶链式反应证实了这些miRNAs的表达。这六种miRNAs在海马区的表达水平明显高于其他组织，包括大脑皮层、心脏、肝脏、肺和肾脏。MiR-384*在大鼠海马区的表达是miR-384的10倍，但miR-330*和miR-330在同一组织中的表达差异不大。
MicroRNAs (miRNAs) are small regulatory molecules post-transcriptionally suppressing mRNA activity. Many miRNAs in various organisms have been cloned but many unknown miRNAs remain to be identified. Here we describe the cloning of six new miRNAs from rat hippocampus. Among them, four were not found in the rat miRBase, but were identical to their human and/or mouse homolog, therefore they were designated as rno-miR-92b, rno-miR-146b, rno-let-7g, and rno-miR-551b. The other two were derived from the other arms of the known miRNA precursors of rno-miR-330 and rno-miR-384, and were not found in miRBase of all organisms. They were designated as rno-miR-330* and rno-miR-384*. The expression of these miRNAs was confirmed by RNA-tailing and primer-extension real-time reverse transcription-polymerase chain reaction. These six miRNAs were expressed at significantly higher levels in the hippocampus than in other tissues, including cerebral cortex, heart, liver, lung and kidney. miR-384* was 10 times more abundant than miR-384 in rat hippocampus, but little difference was found between miR-330* and miR-330 expression in the same tissue.