DIFFERENTIAL DISTRIBUTION OF PARVALBUMIN-IMMUNOREACTIVE PERICELLULAR CLUSTERS OF TERMINAL BOUTONS IN DEVELOPING AND ADULT MONKEY NEOCORTEX
DIFFERENTIAL DISTRIBUTION OF PARVALBUMIN-IMMUNOREACTIVE PERICELLULAR CLUSTERS OF TERMINAL BOUTONS IN DEVELOPING AND ADULT MONKEY NEOCORTEX
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DOI：
10.1016/0014-4886(92)90058-x
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发表时间：
1992-02-01
影响因子：
5.3
通讯作者：
LEWIS, DA
中科院分区：
文献类型：
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作者：
AKIL, M;LEWIS, DA
篮状细胞是gaba能抑制性中间神经元和已知的锥体细胞的调节剂，锥体细胞是新皮层中兴奋性神经元的主要类别。细小白蛋白（PV）是一种钙结合蛋白，在皮质神经元中与GABA共定位（Celio, 1986）。《科学》（science） 231: 995-998)，并且据报道存在于猴新皮层中形成细胞周围簇的篮状神经元的末端钮扣中（Hendryet .1989. exp .）。脑科学，76:467-472)。在这项研究中，我们使用免疫组织化学方法评估了新生儿、婴儿、青少年和成年恒河猴新皮层中pv免疫反应性（PV-IR）终端钮扣细胞周围簇的区域和层状分布。PV-IR细胞周围簇由标记的末端扣组成，这些末端扣勾勒出初级运动皮层第III层和第V层、初级视觉皮层第V层和第VI层以及视觉关联皮层第V层（18区）的大锥体神经元的体细胞和近端树突。这种层流模式在新生动物中存在，并且不随年龄变化而改变。然而，在青春期和成年动物的视觉区域中，没有检测到这种PV-IR结构。在任何年龄的前额皮质均未观察到pv阳性的细胞周围簇。含有pv的细胞周围簇的分布模式与含有非磷酸化神经丝蛋白（NFP）的锥体神经元亚群的分布模式相似；双标记研究证实，一个亚组的nfp阳性锥体神经元是PV-IR细胞周围簇的目标。将PV-IR细胞周围簇的分布与另一类中间神经元-吊灯细胞的PV-IR终端钮扣的分布进行了比较。枝形神经元轴突的末端钮扣排列成垂直的杆状结构，称为脑筒。枝形轴突筒的亚群先前已被证明是PV-IR，并且它们在视觉和前额皮质中的分布已被描述（DeFelipeet al.1989）。中国生物医学工程学报（英文版）。[j]。[j] .中国生物医学工程学报。2003(3):599-615。这两种由PV-IR终端扣组成的结构往往存在于所有区域和年龄的不同层中，除了在初级运动皮层的第三层，在那里发现了PV-IR细胞周围簇和吊灯筒。这些发现表明，在猴新皮层中，PV免疫反应存在于可能由篮状细胞产生的末端钮扣的细胞周围簇中。这些结构中PV免疫反应性的不同区域、层流和发育模式可能揭示其独特的锥体细胞靶点，并为其在猴新皮层中的功能作用提供见解。
Basket cells are GABAergic inhibitory interneurons and known regulators of pyramidal cells, the major class of excitatory neurons in neocortex. Parvalbumin (PV), a calcium binding protein, has been colocalized with GABA in cortical neurons (Celio, 1986.Science231: 995–998) and has been reported to be present in the terminal boutons of basket neurons forming pericellular clusters in monkey neocortex (Hendryet al.1989.Exp. Brain Res.76: 467–472). In this study, we used immunohistochemical methods to evaluate the regional and laminar distributions of PV-immunoreactive (PV-IR) pericellular clusters of terminal boutons in the neocortex of neonatal, infant, adolescent, and adult rhesus monkeys. PV-IR pericellular clusters were composed of labeled terminal boutons that outlined the somata and proximal dendrites of large pyramidal neurons in layers III and V of primary motor cortex, layers V and VI of primary visual cortex, and layer V of visual association cortex (area 18). This laminar pattern was present in neonatal animals and did not change with age in motor cortex. However, in the visual regions of adolescent and adult animals, such PV-IR structures were not detected. PV-positive pericellular clusters were not observed in the prefrontal cortex at any age. The pattern of distribution of PV-containing pericellular clusters paralleled that of a subpopulation of pyramidal neurons containing nonphosphorylated neurofilament proteins (NFP); double labeling studies confirmed that a sub-group of NFP-positive pyramidal neurons were the targets of PV-IR pericellular clusters. The distribution of PV-IR pericellular clusters was compared to that of PV-IR terminal boutons of another class of interneurons, the chandelier cells. Terminal boutons of chandelier neuron axons align in vertical rod-like structures known as cartridges. Subpopulations of chandelier axon cartridges have been previously shown to be PV-IR and their distribution in visual and prefrontal cortices has been described (DeFelipeet al.1989.Brain Res.503: 49–54; Lewis and Lund. 1990.J. Comp. Neurol.293: 599–615). These two types of structures composed of PV-IR terminal boutons tended to be present in different laminae in all regions and ages examined, except in layer III of primary motor cortex where both PV-IR pericellular clusters and chandelier cartridges were found. These findings indicate that in monkey neocortex PV immunoreactivity is present in pericellular clusters of terminal boutons that are likely to arise from basket cells. The differential regional, laminar and developmental patterns of PV immunoreactivity in these structures may reveal their unique pyramidal cell targets and provide insight into their functional roles in monkey neocortex.