Molecular Characterization of Fluoroquinolone-Resistant Moraxella catarrhalis Variants Generated In Vitro by Stepwise Selection
Molecular Characterization of Fluoroquinolone-Resistant Moraxella catarrhalis Variants Generated In Vitro by Stepwise Selection
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通过逐步选择体外产生的耐氟喹诺酮类卡他莫拉菌变种的分子表征
DOI：
10.1128/aac.01336-17
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发表时间：
2017
影响因子：
4.9
通讯作者：
Fujisaki Shingo
中科院分区：
文献类型：
--
作者：
Yamada Kageto;Saito Ryoichi;Muto Saori;Kashiwa Machiko;Tamamori Yoshiko;Fujisaki Shingo
卡他莫拉菌会引起呼吸道感染。本研究以氟喹诺酮类耐药菌株为研究对象，探讨其耐药机制。通过对左氧氟沙星的逐步筛选，获得氟喹诺酮类药物敏感性降低的菌株，并对氟喹诺酮类药物靶点进行测序。6个新的突变GyrA(D84Y、T594dup和A722dup)、GyrB(E479K和D439N)和PARE(Q395R)与卡他毛念珠菌对氟喹诺酮类药物的耐药性有关。
Moraxella catarrhalis causes respiratory infections. In this study, fluoroquinolone-resistant strains were selectedin vitroto evaluate the mechanism of fluoroquinolone resistance. Strains with reduced fluoroquinolone susceptibility were obtained by stepwise selection in levofloxacin, and fluoroquinolone targetsgyrandparwere sequenced. Six novel mutations in GyrA (D84Y, T594dup, and A722dup), GyrB (E479K and D439N), and ParE (Q395R) involved in M. catarrhalis resistance to fluoroquinolones were revealed.