Comparison of resting egg gene expression with different hatchability related to salinity variations in the marine rotifer Brachionus manjavacas
Comparison of resting egg gene expression with different hatchability related to salinity variations in the marine rotifer Brachionus manjavacas
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海洋轮虫 Brachionus manjavacas 与盐度变化相关的不同孵化率的静息卵基因表达比较
DOI：
10.1007/s12562-018-1213-6
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发表时间：
2018
影响因子：
1.9
通讯作者：
Atsushi Hagiwara
中科院分区：
文献类型：
--
作者：
Chengyan Han;Hee-Jin Kim;Koushirou Suga;Mingyou Li;Atsushi Hagiwara
盐度是影响广盐性轮虫休眠卵孵化的重要因素。为了阐明盐度对轮虫休眠卵孵化率的影响，研究了两种不同盐度（17和33 ppt）孵育条件下，轮虫休眠卵基因表达的变化。孵化盐度为17 ppt时，休眠卵的孵化率高于33 ppt。与这些情况相关，在17 ppt下孵育的休眠卵表达具有暗示细胞分化和胚胎发育的推定功能的基因：晚期胚胎发生丰富蛋白（LEAs-1）、α-淀粉酶和脱氨酶。33 ppt孵育的休眠卵高表达与环境胁迫相关的基因：AP 2转录因子（AP 2 TF）和ATP分解相关基因：ABC转运通透酶（ABC-TP）、NAD +合酶、铜转运P型ATP酶（CTP-ATP酶）。预计在33 ppt下孵育的休眠卵可能需要更多的能量（ATP）来承受孵育期间的盐胁迫。结果表明，休眠卵通过在胚胎发育和对环境条件（如盐度胁迫）的自我防御之间分配能量来调节其孵化。
Salinity is a significant factor in affecting resting egg hatching in the euryhaline rotifers. In order to clarify the effects of salinity on resting egg hatchability, this study investigated gene expressions of resting eggs subjected to two different incubation salinities (at 17 and 33 ppt) in the rotiferBrachionus manjavacas. The resting eggs formed at 17 ppt showed a higher hatching rate at 17 ppt incubation salinity, compared to those at 33 ppt. Related to these circumstances, the resting eggs incubated at 17 ppt expressed genes which have putative functions implying cellular differentiation and embryonic development: late embryogenesis abundant protein (LEAs-1), α-amylase, and deaminase. The resting eggs incubated at 33 ppt highly expressed the genes related to the environmental stresses: AP2 transcription factors (AP2TF), and ATP decomposition: ABC transporter permease (ABC-TP), NAD + synthase, copper-translocating P-type ATPase (CTP-ATPase). It is expected that the resting eggs incubated at 33 ppt may need more energy (ATP) to endure saline stress during incubation. The obtained results indicated that the resting eggs regulate their hatching by allocating energy between embryo development and self-defense against environmental conditions like salinity stresses.