Organization of cells and extracellular matrix in mesenteric arteries of spontaneously hypertensive rats.
Organization of cells and extracellular matrix in mesenteric arteries of spontaneously hypertensive rats.
复制标题
自发性高血压大鼠肠系膜动脉中细胞和细胞外基质的组织。
DOI：
10.1007/bf00384734
复制
发表时间：
1992
影响因子：
3.6
通讯作者：
McGuffee,LJ
中科院分区：
文献类型：
--
作者：
Walker-Caprioglio,HM;Trotter,JA;Little,SA;McGuffee,LJ
生化研究已被用来评估弹性蛋白和胶原蛋白在高血压与正常血压动脉的定量变化。然而，这些纤维蛋白质的相对分布和组织可能与它们的绝对量同等重要。在这项研究中，我们已经使用扫描电子显微镜与选择性消化技术，以评估自发性高血压大鼠肠系膜动脉的图尼卡中膜的细胞和细胞外成分的组织。分别用酸、碱或漂白剂消化上级和小肠系膜动脉以暴露细胞、胶原蛋白或胶原蛋白和弹性蛋白。我们观察到，与血压正常的动脉相比，高血压不会引起自发性高血压动脉中细胞、胶原蛋白或弹性蛋白的三维排列发生质的变化。然而，当与小动脉的细胞相比时，上级动脉中的细胞在总体形状和表面特征上显著不同。细胞表面形态的这些差异存在于高血压和血压正常的血管中，并表明上级和小肠系膜动脉细胞以不同的方式将负荷传递到各向同性基质。在弹性肌性上级动脉中，力通过指状结构传递到整个细胞表面。在肌性小动脉中，力通过锥形光滑的细胞表面传递。
Biochemical studies have been used to assess the quantitative changes in elastin and collagen in hypertensive vs. normotensive arteries. However, the relative distribution and organization of these fibrous proteins is likely to be equal in importance to their absolute amounts. In this study we have used scanning electron microscopy in association with selective digestion techniques to assess the organization of cellular and extracellular components of the tunica media of mesenteric arteries of spontaneously hypertensive rats. Superior and small mesenteric arteries were digested with acid, alkali, or bleach to exposure cells, collagen, or collagen and elastin, respectively. We observed that hypertension does not cause a qualitative change in the 3-dimensional arrangement of cells, collagen, or elastin in spontaneously hypertensive arteries when compared to normotensive arteries. However, cells in the superior artery are significantly different in overall shape and surface features when compared to cells of small arteries. These differences in surface morphology of cells are present in hypertensive and normotensive vessels and suggest that superior and small mesenteric artery cells transmit load to the isotropic matrix in different ways. In the elasto-muscular superior artery, force is transmitted across digitations throughout the cell surface. In the muscular small artery, force is transmitted across the tapered, smooth cell surface.