Sodium butyrate, a histone deacetylase inhibitor, regulates Lymphangiogenic factors in oral cancer cell line HSC-3.
Sodium butyrate, a histone deacetylase inhibitor, regulates Lymphangiogenic factors in oral cancer cell line HSC-3.
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DOI：
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发表时间：
2014-04
影响因子：
2
通讯作者：
T. Yamamura;N. Matsumoto;Yasuyoshi Matsue;Michisato Okudera;Y. Nishikawa;Y. Abiko;K. Komiyama
中科院分区：
文献类型：
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作者：
T. Yamamura;N. Matsumoto;Yasuyoshi Matsue;Michisato Okudera;Y. Nishikawa;Y. Abiko;K. Komiyama
目的肿瘤血管生成是分子靶向治疗的研究热点。本研究探讨丁酸钠（SB），一种组蛋白去乙酰化酶抑制剂，对口腔鳞状细胞癌中抗血管生成因子和淋巴管生成因子合成的影响。设计应用cDNA微阵列技术检测SB处理前后HSC-3细胞基因的变化，应用定量PCR、免疫印迹和免疫细胞化学技术检测淋巴管生成因子mRNA和蛋白的表达。结果芯片分析显示SB处理导致血管生成相关基因表达的改变。定量聚合酶链反应显示，血小板衍生生长因子-B，血管生成素-2，血管内皮生长因子（VEGF）-C，和VEGFD下调。Western印迹和免疫细胞化学证实了VEGFC的蛋白质合成减少。结论SB抑制HSC-3细胞淋巴管生成因子的表达。在本研究的限制范围内，SB可能具有作为口腔癌抗转移前药的潜力。
AIM Tumor angiogenesis is a focus of molecularly-targeted therapies. This study investigated the effect of sodium butyrate (SB), a histone deacetylase inhibitor, on the synthesis of antiangiogenic and lymphangiogenic factors in oral squamous cell carcinoma. DESIGN Gene alterations in HSC-3 cells were assessed using cDNA microarrays before and after treatment with SB. The mRNA and protein expression of lymphangiogenic factors were also assessed by quantitative PCR, western blotting and immunocytochemistry. RESULTS Microarray analysis revealed that treatment with SB led to altered expression of angiogenesis-related gene expression. The quantitative polymerase chain reaction showed that platelet-derived growth factor-B, angiopoietin-2, vascular endothelial growth factor (VEGF)-C, and VEGFD were down-regulated. Western blotting and immunocytochemistry confirmed reduced protein synthesis of VEGFC. CONCLUSION SB inhibits expression of lymphangiogenic factors in HSC-3 cells. Within the limitations of the present study, SB may have potential as an anti-metastatic pro-drug for oral cancer.