Antigen-induced monocyte procoagulant activity. Requirement for antigen presentation and histocompatibility leukocyte antigen-DR molecules.
Antigen-induced monocyte procoagulant activity. Requirement for antigen presentation and histocompatibility leukocyte antigen-DR molecules.
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抗原诱导的单核细胞促凝血活性。
DOI：
10.1172/jci112097
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Schwartz,BS
中科院分区：
文献类型：
--
作者：
Schwartz,BS
本研究探讨了淋巴细胞和单核细胞之间的相互作用，单核细胞在对结核菌（PPD）或兔热病抗原纯化蛋白衍生物的反应中表达促凝活性（PCA）。PCA反应是抗原特异性的：对PPD或兔贫血敏感的供者外周血单个核细胞（PBM）仅在致敏抗原反应中显示PCA增加。PCA是组织因子样的，表达活性需要因子VII和因子X，而因子VIII则不需要。抗原刺激后PBM分化为淋巴细胞和单核细胞，结果表明超过90%的PCA与单核细胞相关。与致敏抗原孵育的分离单核细胞或淋巴细胞与对照细胞具有相同的PCA。纯化的淋巴细胞经抗原脉冲后，不能引起加入抗原的单核细胞的PCA反应。然而，与抗原孵育的贴壁单核细胞，然后洗去未结合的蛋白，能够触发淋巴细胞成为PCA对应答单核细胞的刺激。在培养过程中加入一种针对HLA-DR抗原的单克隆抗体，可以阻断PBM中抗原特异性PCA的发展。该抗体对凝血试验、预成型PCA、细胞活力或刺激抗原本身没有影响。这些结果表明，单核细胞对PCA的阐述可能是经典抗原免疫反应的固有部分，并可能解释免疫病变中纤维蛋白的存在，以及许多免疫疾病中血栓性并发症的发生。
The present study explores the interactions between lymphocytes and monocytes that are required for expression of procoagulant activity (PCA) by monocytes in response to purified protein derivative of the tubercle bacillus (PPD) or tularemia antigen. The PCA response was antigen specific: peripheral blood mononuclear cells (PBM) from donors sensitive to PPD or tularemia showed an increase in PCA only in response to the sensitizing antigen. The PCA was tissue factorlike in that Factors VII and X were required for expression of the activity, whereas Factor VIII was not. Maximum PCA developed only after 36 to 72 h. Fractionation of PBM into lymphocytes and monocytes after antigenic stimulation demonstrated that greater than 90% of the PCA was associated with monocytes. Isolated monocytes or lymphocytes incubated with sensitizing antigen had the same PCA as control cells. Purified lymphocytes that had been pulsed with antigen were unable to elicit a PCA response from monocytes to which they were added. However, adherent monocytes incubated with antigen, then washed free of unbound protein, were able to trigger lymphocytes to become stimulatory for PCA toward responding monocytes. The development of antigen-specific PCA in PBM could be blocked by including a monoclonal antibody to HLA-DR antigen in the incubation. The antibody had no effect on the clotting assay, on preformed PCA, cell viability, or on stimulatory antigen itself. These results indicate that elaboration of PCA by mononuclear cells may be an intrinsic part of the classical immune response to antigen, and may explain the presence of fibrin in immune lesions, as well as the occurrence of thrombotic complications in many immune disorders.