Discrimination between wild-type and ampicillin-resistant Escherichia coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
Discrimination between wild-type and ampicillin-resistant Escherichia coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
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DOI：
10.1007/s00216-007-1558-7
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发表时间：
2007-11-01
影响因子：
4.3
通讯作者：
Hays, Faith A.
中科院分区：
文献类型：
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作者：
Camara, Johanna E.;Hays, Faith A.
基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）进行了优化，以区分野生型和氨苄青霉素耐药大肠杆菌。只有氨苄青霉素耐药的E.大肠杆菌显示出m/z约为29，000的峰，通过胶内消化和随后的肽质量指纹分析证实其为β-内酰胺酶。快速MALDI-TOF MS检测耐药性可以满足重要的临床需求，提供超越属种鉴定的关键表型信息。
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was optimized to discriminate between wild-type and ampicillin-resistant Escherichia coli. Only ampicillin-resistant E. coli displayed an m/z approximate to 29,000 peak, which was confirmed as beta-lactamase by in-gel digestion followed by peptide mass fingerprinting. Rapid MALDI-TOF MS detection of antibiotic-resistance could fulfill an important clinical need, providing critical phenotypic information beyond genus-species identification.