Occurrence of ESBL-Producing Escherichia coli in Livestock and Farm Workers in Mecklenburg-Western Pomerania, Germany.
Occurrence of ESBL-Producing Escherichia coli in Livestock and Farm Workers in Mecklenburg-Western Pomerania, Germany.
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DOI：
10.1371/journal.pone.0143326
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Kramer A
中科院分区：
文献类型：
--
作者：
Dahms C;Hübner NO;Kossow A;Mellmann A;Dittmann K;Kramer A
近年来，在家畜中发现了广谱β-内酰胺酶（ESBL）产生菌，主要是无症状的定殖菌。与畜牧业密切接触的工作人员的人畜共患风险仍未得到完全评估。因此，我们调查了牲畜和工人中产生esbls的埃希氏菌的流行情况，以确定动物-人交叉传播的潜在风险。在德国东北部的梅克伦堡-西波美拉尼亚州，对来自23个不同农场的73名与牲畜接触的人进行了腹沟拭子检测，以检测产生esbls的埃希氏菌。对来自34个不同农场（17个猪场，11个牛场，6个家禽农场）的动物来源的每个农场采集了两份粪便样本，并在每个家禽农场随机抽取了10只肉鸡或火鸡的肛肠拭子。为了鉴定，在富集步骤后使用选择性显色琼脂。利用PCR技术检测产生esbl表型的分离株（n = 99）的CTX-M、OXA、SHV和TEM，并利用多位点序列分型（MLST）进一步对分离株进行鉴定。共获得61株不同来源的菌株和/或不同的MLST/PCR结果。5名农场工人（3名来自养牛场，2名来自养猪场）携带产生esbl的大肠杆菌。所有人分离株均含有CTX-M β-内酰胺酶；在两种样品中分别检测到TEM和OXA β-内酰胺酶。1、隔离。在猪（15/17）、牛（6/11）和家禽（3/6）的粪便样本中发现产esbl的埃希氏菌。共有70.6%（24/36）的养殖场ESBL阳性。此外，60例直肠拭子中有9例为ESBL阳性。从动物源分离到的产esbl细菌除一株赫曼氏大肠杆菌外均为大肠杆菌。牛和猪场中最常见的β-内酰胺酶是CTX-M，而家禽中以SHV为主。1例人类分离株与同一农场牛粪便样本中发现的分离株具有相同的MLST序列型（ST） 3891和CTX-M等位基因，表明存在人畜共患转移。另外两对人-猪和人-牛大肠杆菌分离株编码相同的ESBL基因，但不具有相同的MLST，这可能表明水平抗性基因转移。总而言之，该研究表明，梅克伦堡-西波美拉尼亚的牲畜中产生esbls的大肠杆菌的流行率很高，并提供了牲畜和农场工人之间转移的风险。
In recent years, extended-spectrum β-lactamases (ESBL) producing bacteria have been found in livestock, mainly as asymptomatic colonizers. The zoonotic risk for people working in close contact to animal husbandry has still not been completely assessed. Therefore, we investigated the prevalence of ESBL-producing Escherichia spp. in livestock animals and workers to determine the potential risk for an animal-human cross-transmission.In Mecklenburg-Western Pomerania, northeast Germany, inguinal swabs of 73 individuals with livestock contact from 23 different farms were tested for ESBL-producing Escherichia spp. Two pooled fecal samples per farm of animal origin from 34 different farms (17 pig farms, 11 cattle farms, 6 poultry farms) as well as cloacal swabs of 10 randomly selected broilers or turkeys were taken at each poultry farm. For identification, selective chromogenic agar was used after an enrichment step. Phenotypically ESBL-producing isolates (n = 99) were tested for CTX-M, OXA, SHV and TEM using PCR, and isolates were further characterized using multilocus sequence typing (MLST). In total, 61 diverse isolates from different sources and/or different MLST/PCR results were acquired. Five farm workers (three from cattle farms and two from pig farms) harbored ESBL-producing E. coli. All human isolates harbored the CTX-M β-lactamase; TEM and OXA β-lactamases were additionally detected in two, resp. one, isolates. ESBL-producing Escherichia spp. were found in fecal samples at pig (15/17), cattle (6/11) and poultry farms (3/6). In total, 70.6% (24/36) of the tested farms were ESBL positive. Furthermore, 9 out of 60 cloacal swabs turned out to be ESBL positive. All isolated ESBL-producing bacteria from animal sources were E. coli, except for one E. hermanii isolate. CTX-M was the most prevalent β-lactamase at cattle and pig farms, while SHV predominated in poultry. One human isolate shared an identical MLST sequence type (ST) 3891 and CTX-M allele to the isolate found in the cattle fecal sample from the same farm, indicating a zoonotic transfer. Two other pairs of human-pig and human-cattle E. coli isolates encoded the same ESBL genes but did not share the same MLST ST, which may indicate horizontal resistance gene transfer. In summary, the study shows the high prevalence of ESBL-producing E.coli in livestock in Mecklenburg- Western Pomerania and provides the risk of transfer between livestock and farm workers.