IL-4 is essential for the systemic transfer of delayed hypersensitivity by T cell lines. Role of gamma/delta cells.
IL-4 is essential for the systemic transfer of delayed hypersensitivity by T cell lines. Role of gamma/delta cells.
复制标题
IL-4 对于 T 细胞系迟发性超敏反应的全身转移至关重要。
作者：
F. Dieli;G. Asherson;G. Romano;G. Sireci;F. Gervasi;A. Salerno
体外用抗原反复刺激免疫小鼠的淋巴细胞，获得半抗原(三硝基苯基)特异性T细胞系。这些T细胞株表现出典型的Th1细胞的表型特征和细胞因子的产生模式，由90%以上的Vβ8.2+T淋巴细胞和6%至9%的伽马/Delta+T淋巴细胞组成。当注射到抗原攻击部位时，这些线介导了DTH的局部被动转移，但当静脉注射时，它们未能介导DTH的全身性被动转移。然而，当IL-4与T细胞系一起给予受体小鼠或当T细胞系与IL-4体外孵育时，观察到成功的系统被动转移DTH。IL-4能在体外和体内分别以10pg/ml和10pg/只的剂量实现全身性、特异性的DTH被动转移；在细胞转移前4h注射IL-4是有效的，但提前1-5天给药无效。细胞荧光分析表明，该品系中的γ/Delta+细胞表达IL-4R，而Vβ8.2+细胞则表达IL-4R，当γ/Delta+细胞与IL-4体外孵育并与Vβ8.2+细胞一起注射到受体小鼠体内时，可以观察到DTH的良好全身转移。相反，注射经IL-4处理的Vβ8.2+细胞和γ/Delta+细胞不能转移迟发型超敏反应。综上所述，目前的结果表明，IL-4是DTH反应中的一个重要介质，而γ/Delta+细胞是其作用靶点之一。
Hapten (trinitrophenyl)-specific T cell lines were obtained by repeated stimulation of lymph node cells from immune mice with Ag in vitro. These T cell lines show phenotypic properties and a pattern of cytokine production typical of Th1 cells and consisted of more than 90% V beta 8.2+ T lymphocytes and 6 to 9% gamma/delta + T lymphocytes. The lines mediate a local passive transfer of DTH when injected at the site of Ag challenge but fail to mediate a systemic passive transfer of DTH when injected i.v. However, a successful systemic passive transfer of DTH was observed when IL-4 was given to recipient mice together with the T cell lines or when the T cell lines were incubated in vitro with IL-4. IL-4 enables systemic, specific passive transfer of DTH at a dose of 10 pg/ml in vitro and at a dose of 10 pg/mouse in vivo; it is effective when injected 4 h before cell transfer but not when given 1 to 5 days earlier. Cytofluorimetric analysis shows that the gamma/delta + cells and not the V beta 8.2+ cells of the line express IL-4R and a good systemic transfer of DTH is observed when gamma/delta + cells are incubated in vitro with IL-4 and then injected together with V beta 8.2+ cells into recipient mice. In contrast, injection of V beta 8.2+ cells treated with IL-4 together with gamma/delta + cells fails to transfer DTH. Overall, the present results show that IL-4 is an important mediator in the DTH reaction and that gamma/delta + cells are one of the targets of its action.