Suppression of tumor-induced angiogenesis by Brazilian propolis: Major component artepillin C inhibits in vitro tube formation and endothelial cell proliferation
Suppression of tumor-induced angiogenesis by Brazilian propolis: Major component artepillin C inhibits in vitro tube formation and endothelial cell proliferation
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DOI：
10.1016/j.canlet.2006.12.039
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发表时间：
2007-07-18
期刊：
影响因子：
9.7
通讯作者：
Nakayama, Tsutomu
中科院分区：
文献类型：
--
作者：
Ahn, Mok-Ryeon;Kunimasa, Kazuhiro;Nakayama, Tsutomu
蜂胶是蜜蜂从各种植物中采集的一种重要的生物活性物质，具有抗肿瘤等多种生理活性。我们以前已经表明，巴西原产地的青蒿素主要由artepillin C组成，其成分与欧洲原产地的青蒿素完全不同。在这份报告中，我们研究了巴西阿替匹林的抗血管生成作用，并研究了阿替匹林C是否对这种作用负责。在使用ICR小鼠的体内血管生成试验中，我们发现巴西枸杞的乙醇提取物（EEBP）显着减少了新形成的血管的数量。EEBP在体外试管形成试验中也显示出抗血管生成作用。当与EEBP的其他成分相比时，发现只有artepillin C以浓度依赖性方式（3.13-50 μ g/ml）显著抑制HUVEC的管形成。此外，阿替匹林C以浓度依赖性方式（3.13-50 μ g/ml）显著抑制HUVECs的增殖。此外，在体内血管生成测定中，阿替匹林C显著减少了新形成的血管的数量。从其在体外和体内的抗血管生成活性，我们得出结论，阿替匹林C至少部分负责EEBP在体内的抗血管生成活性。阿替匹林C可能被证明可用于开发对肿瘤血管生成具有治疗或预防活性的药物和食品。(c)2007爱思唯尔爱尔兰有限公司保留所有权利。
Propolis, a resinous substance collected by honeybees from various plant sources, possesses various physiological activities such as antitumor effects. We have previously shown that propolis of Brazilian origin was composed mainly of artepillin C and that its constituents were quite different from those of propolis of European origin. In this report, we examined an antiangiogenic effects of Brazilian propolis and investigated whether artepillin C was responsible for such effects. In an in vivo angiogenesis assay using ICR mice, we found that the ethanol extract of Brazilian propolis (EEBP) significantly reduced the number of newly formed vessels. EEBP also showed antiangiogenic effects in an in vitro tube formation assay. When compared with other constituents of EEBP, only artepillin C was found to significantly inhibit the tube formation of HUVECs in a concentration-dependent manner (3.13-50 mu g/ml). In addition, artepillin C significantly suppressed the proliferation of HUVECs in a concentration-dependent manner (3.13-50 mu g/ml). Furthermore, artepillin C significantly reduced the number of newly formed vessels in an in vivo angiogenesis assay. Judging from its antiangiogenic activity in vitro and in vivo, we concluded that artepillin C at least in part is responsible for the antiangiogenic activity of EEBP in vivo. Artepillin C may prove useful in the development of agents and foods with therapeutic or preventive activity against tumor angiogenesis. (c) 2007 Elsevier Ireland Ltd. All rights reserved.