Identification of microRNAs and Their Target Genes Explores miRNA-Mediated Regulatory Network of Cytoplasmic Male Sterility Occurrence during Anther Development in Radish (Raphanus sativus L.).
Identification of microRNAs and Their Target Genes Explores miRNA-Mediated Regulatory Network of Cytoplasmic Male Sterility Occurrence during Anther Development in Radish (Raphanus sativus L.).
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DOI：
10.3389/fpls.2016.01054
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发表时间：
2016
影响因子：
5.6
通讯作者：
Liu L
中科院分区：
文献类型：
--
作者：
Zhang W;Xie Y;Xu L;Wang Y;Zhu X;Wang R;Zhang Y;Muleke EM;Liu L
MicroRNAs（miRNAs）是一类内源性非编码小分子RNA，在植物生长发育过程中起着重要作用。细胞质雄性不育是一种典型的母系遗传性状，在植物杂种优势利用中有着广泛的应用。然而，萝卜花药发育过程中细胞质雄性不育发生的miRNA介导的调控网络在很大程度上仍然未知。本研究利用高通量测序技术对雄性不育系WA及其保持系WB的花蕾进行了比较小RNA组测序。在萝卜花芽中共分离到162个已知的miRNA，分别属于25个保守的miRNA家族和24个非保守的miRNA家族，并首次鉴定出27个新的miRNA家族。在这些miRNAs中，28个已知的和14个潜在的新的miRNAs在花药发育过程中差异表达。与CMS发生相关的miRNAs靶基因编码重要的转录因子和功能蛋白，可能参与生长素信号转导、信号转导、miRNAs靶沉默、花器官发育和细胞器基因表达等多种生物学过程。此外，还利用qRT-PCR技术分析了几种与CMS发生相关的miRNAs及其靶基因在花药发育3个阶段的表达模式。此外，首次提出了萝卜花药发育过程中可能存在的由miRNA介导的CMS发生调控网络。这些研究结果将为深入了解miRNA介导的CMS发生的复杂遗传调控网络提供新的视角，并进一步加深我们对miRNA在萝卜等作物CMS发生和小孢子形成中的作用的理解。
MicroRNAs (miRNAs) are a type of endogenous non-coding small RNAs that play critical roles in plant growth and developmental processes. Cytoplasmic male sterility (CMS) is typically a maternally inherited trait and widely used in plant heterosis utilization. However, the miRNA-mediated regulatory network of CMS occurrence during anther development remains largely unknown in radish. In this study, a comparative small RNAome sequencing was conducted in floral buds of CMS line ‘WA’ and its maintainer line ‘WB’ by high-throughput sequencing. A total of 162 known miRNAs belonging to 25 conserved and 24 non-conserved miRNA families were isolated and 27 potential novel miRNA families were identified for the first time in floral buds of radish. Of these miRNAs, 28 known and 14 potential novel miRNAs were differentially expressed during anther development. Several target genes for CMS occurrence-related miRNAs encode important transcription factors and functional proteins, which might be involved in multiple biological processes including auxin signaling pathways, signal transduction, miRNA target silencing, floral organ development, and organellar gene expression. Moreover, the expression patterns of several CMS occurrence-related miRNAs and their targets during three stages of anther development were validated by qRT-PCR. In addition, a potential miRNA-mediated regulatory network of CMS occurrence during anther development was firstly proposed in radish. These findings could contribute new insights into complex miRNA-mediated genetic regulatory network of CMS occurrence and advance our understanding of the roles of miRNAs during CMS occurrence and microspore formation in radish and other crops.