Metabolomic analysis of energy regulated germination and sprouting of organic mung bean (Vigna radiata) using NMR spectroscopy
Metabolomic analysis of energy regulated germination and sprouting of organic mung bean (Vigna radiata) using NMR spectroscopy
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DOI：
10.1016/j.foodchem.2019.01.183
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发表时间：
2019-07-15
期刊：
影响因子：
8.8
通讯作者：
Yang, Hongshun
中科院分区：
文献类型：
--
作者：
Chen, Lin;Wu, Ji'en;Yang, Hongshun
发芽和发芽受能量状态的调节。用三磷酸腺苷和2，4-二硝基苯酚（DNP）处理绿豆种子，研究了不同浓度的DNP对绿豆种子萌发的影响。研究了不同能量状态下绿豆发育过程中的代谢组学变化。总共鉴定了42种代谢物。主成分分析表明，种子中产生的特征化合物是油酸，亚油酸和琥珀酸。糖，包括麦芽糖，蔗糖和葡萄糖与发芽有关。绿豆种子利用不同的能源，并产生较高的琥珀酸含量。糖和次生代谢产物在芽中积累。氮、糖和氨基酸代谢途径参与了这一生理过程。DNP引起能量不足，这导致葡萄糖的消耗和翻译。其它氨基酸和氨基酸含量较高。转录结果进一步证实了我们的代谢假说。因此，充足的能量供应是芽发育和营养代谢产物合成的关键。
Germination and sprouting are regulated by the energy status. In the present study, mung bean seeds were treated with adenosine triphosphate and 2,4-dinitrophenol (DNP). The metabolomic changes during development of mung beans under different energy statuses were investigated. In total, 42 metabolites were identified. Principal component analysis revealed that the featured compounds produced in seeds were oleic, linoleic, and succinic acids. Sugars, including maltose, sucrose, and glucose were related to sprouting. Mung bean seeds utilised diverse energy resources and produced higher succinic acid content. Sugars and secondary metabolites accumulated in sprouts. Nitrogen, sugar, and amino acid metabolism pathways contributed to this physiological process. DNP caused an energy deficit, which resulted in the consumption and translation of glucose. Higher contents of other saccharides and amino acids were observed. The transcriptional results further confirmed our metabolic hypothesis. In conclusion, sufficient energy supply is crucial for sprout development and nutritive metabolite synthesis.